电针通过下调慢性脑缺血大鼠Ang II/AT1R/NOX轴抑制氧化应激，改善认知功能。

IF 2.5 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-02-23 DOI:10.1016/j.neulet.2025.138179

Qi Ai , Jurui Wei , Bijun Luo , Zixuan Wang , Jun Dong , Jiumei Zhao , Wenqing Xu , Lin Chen , Wenyao Fang , Songjie Zhu

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引用次数: 0

摘要

目的：探讨电针对慢性脑缺血（CCI）的影响。方法：采用颈总动脉阻断法建立CCI大鼠模型。在模型大鼠的“百会”（GV20）和“大椎”（GV14）穴和/或ARB处进行EA治疗。测定大鼠动脉和海马组织中Ang II和AT1R的表达。免疫组织化学染色检测RECA-1在海马组织中的表达。检测NOX2、NOX4、SOD1、SOD2、ROS、MDA水平。Morris水迷宫法和TUNEL染色法分别探讨EA对认知功能障碍和细胞凋亡的影响。结果：EA降低了CCI大鼠的Ang II和AT1R水平。RECA-1在模型大鼠中表达降低，EA和ARB使其表达升高。EA对CCI大鼠氧化应激有抑制作用。此外，EA还能改善CCI大鼠的认知功能障碍。EA对CCI大鼠海马组织凋亡有抑制作用。此外，抑制Ang II/AT1R/NOX轴可促进EA对模型大鼠氧化应激和认知功能障碍的治疗作用。结论：电针治疗“百会”（GV20）、“大椎”（GV14）穴通过下调Ang II/AT1R/NOX轴对cci诱导的认知功能障碍和氧化应激有明显作用。

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Electroacupuncture inhibits oxidative stress and improves cognitive function by downregulating the Ang II/AT1R/NOX axis in chronic cerebral ischemia rats

Objective

This study aimed to explore the effects of Electroacupuncture (EA) on chronic cerebral ischemia (CCI).

Methods

A CCI rat model was established by blocking the common carotid arteries. Model rats were treated with EA at the “Baihui” (GV20) and “Dazhui” (GV14) acupoints and/or ARB. Ang II and AT1R expression in the artery and hippocampal tissues was determined. Immunohistochemistry staining was used to detect RECA-1 expression in hippocampal tissues. NOX2, NOX4, SOD1, SOD2, ROS, and MDA levels were examined. Morris water maze and TUNEL staining were used to explore the effects of EA on cognitive impairment and apoptosis, respectively.

Results

Ang II and AT1R levels were reduced by EA in CCI rats. RECA-1 expression was reduced in model rats, while EA and ARB increased its expression. EA inhibited oxidative stress in CCI rats. Besides, EA improved cognitive impairment in CCI rats. Apoptosis in the hippocampal tissues of CCI rats was inhibited by EA treatment. Furthermore, inhibition of Ang II/AT1R/NOX axis promoted the therapeutic effects of EA on oxidative stress and cognitive impairment in model rats.

Conclusions

EA treatment at the “Baihui” (GV20) and “Dazhui” (GV14) acupoints is effective against CCI-induced cognitive impairment and oxidative stress by downregulating Ang II/AT1R/NOX axis.

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

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