圈养白掌长臂猿和暹罗猴肠道微生物组评估的高通量DNA元条形码

IF 0.8 4区 农林科学 Q3 VETERINARY SCIENCES
Badrul Munir Md-Zain, Wan Ainin Sofiya Wan-Mustafa, Roberta Chaya Tawie Tingga, Millawati Gani, Abd Rahman Mohd-Ridwan
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引用次数: 0

摘要

肠道微生物群在灵长类动物的整体健康和福祉中起着至关重要的作用,包括小型类人猿(hyloatidae)。细菌和肠道之间的共生关系有助于食物消化,维持宿主健康,并帮助它们适应环境,包括圈养条件。尽管被国际自然保护联盟(IUCN)列为濒危物种红色名录,但与其他灵长类动物相比,对小猿肠道微生物群的分子研究有限。本研究旨在通过评估马来西亚半岛太平动物园和夜间野生动物园圈养小猿的微生物群落,来表征它们的肠道微生物群。方法收集白长臂猿和长臂猿7份粪便标本,分离出其中的细菌并进行16S rRNA基因高通量测序。结果获得性扩增子序列变异(asv)可归属于微生物17门82科164属43种。每只小猿都有独特的肠道菌群。拟杆菌门和厚壁菌门在各个体中均占优势。环境条件和宿主遗传是影响小猿肠道微生物组成的因素之一。结论本研究结果为了解太平动物园和夜间野生动物园小类人猿肠道菌群组成提供了有价值的信息,为圈养小类人猿的健康管理和福利工作提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High-Throughput DNA Metabarcoding for the Gut Microbiome Assessment of Captive White-Handed Gibbon and Siamang

Background

The gut microbiota plays a vital role in primates' overall health and well-being, including small apes (Hylobatidae). The symbiotic relationships between bacteria and the gut aid food digestion, maintain host health, and help them adapt to their environment, including captive conditions. Despite being listed as endangered in the International Union for Conservation of Nature (IUCN) red list category, molecular studies on the small ape's gut microbiome are limited compared to other primates. This study aimed to characterize the gut microbiota of captive small apes at Zoo Taiping and Night Safari, Peninsular Malaysia, by evaluating their microbial communities.

Methods

Seven fecal samples from Hylobatidae (white-handed gibbon and siamang) were collected, and the bacteria therein were successfully isolated and subjected to high-throughput sequencing of the 16S rRNA gene.

Results

The acquired amplicon sequence variants (ASVs) were successfully classified into 17 phyla, 82 families, 164 genera, and 43 species of microbes. Each small ape exhibited a unique gut microbiota profile. The phyla Bacteroidota and Firmicutes were dominant in each individual. Environmental conditions and host genetics are among the factors that influence the small ape's gut microbiome composition.

Conclusions

These findings provide valuable insights into the gut microbiota composition of small apes at Zoo Taiping and Night Safari, thus contributing to the health management and welfare efforts of small apes in captivity.

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来源期刊
CiteScore
1.40
自引率
42.90%
发文量
62
审稿时长
6 months
期刊介绍: The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.
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