Type III Collagen Promotes Pseudopodium-Driven Cell Migration

The extracellular matrix (ECM), particularly collagen, is acknowledged for its significant impact on cell migration. However, the detailed mechanisms through which it influences pseudopodium formation and cell motility are not yet fully understood. This study delves into the impact of recombinant human type III collagen (hCOL3) on cell migration, specifically focusing on the dynamics of pseudopodia and their contribution to cell motility. The research evaluates the impact of a fragmented form of hCOL3, engineered for the study, on cell motility and pseudopodium behavior using both single-cell and collective-cell migration assays. The results demonstrate that hCOL3 promotes cell migration velocity, augments the effective diffusion coefficient, and enhances directionality in both single-cell and collective migration contexts. Observations from scanning electron microscopy reveal that treatment with hCOL3 increases both the number and length of filopodia, which are crucial for cell migration and interaction with the ECM. The study suggests that hCOL3 facilitates a more targeted and rapid migration. The presence of an increased number of filopodia on surfaces treated with hCOL3 enhances the cell’s ability to detect environmental cues and extent, thereby augmenting its migratory capacity. This discovery could potentially lead to greater efficiency in wound healing processes.