{"title":"分化的SH-SY5Y人神经母细胞瘤细胞对1型单纯疱疹病毒和乙型脑炎病毒感染的体外敏感性","authors":"Jannatul Ferdous, Haruka Makino, Tatsunori Masatani, Yoshikazu Fujimoto, Kiyotada Naitou, Mitsuya Shiraishi","doi":"10.1292/jvms.24-0405","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to investigate the effects of cell differentiation status and neurotropic viral infectivity on the susceptibility of undifferentiated and differentiated SH-SY5Y cells to herpes simplex virus type 1 (HSV-1) and Japanese encephalitis virus (JEV) infections. Notably, undifferentiated SH-SY5Y cells were susceptible to both HSV-1 and JEV infections. However, differentiated cells exhibiting a phenotypically mature neuron-like morphology and markedly increased neuronal marker levels showed low susceptibility to JEV infection. Overall, these findings suggest that SH-SY5Y cells become more resistant to JEV infection as they mature. This cell culture model is useful to elucidate the molecular mechanisms regulating the maturation state-specific resistance of neurons to JEV.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"426-430"},"PeriodicalIF":1.1000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964864/pdf/","citationCount":"0","resultStr":"{\"title\":\"In vitro susceptibility of differentiated SH-SY5Y human neuroblastoma cells to herpes simplex virus type 1 and Japanese encephalitis virus infection.\",\"authors\":\"Jannatul Ferdous, Haruka Makino, Tatsunori Masatani, Yoshikazu Fujimoto, Kiyotada Naitou, Mitsuya Shiraishi\",\"doi\":\"10.1292/jvms.24-0405\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to investigate the effects of cell differentiation status and neurotropic viral infectivity on the susceptibility of undifferentiated and differentiated SH-SY5Y cells to herpes simplex virus type 1 (HSV-1) and Japanese encephalitis virus (JEV) infections. Notably, undifferentiated SH-SY5Y cells were susceptible to both HSV-1 and JEV infections. However, differentiated cells exhibiting a phenotypically mature neuron-like morphology and markedly increased neuronal marker levels showed low susceptibility to JEV infection. Overall, these findings suggest that SH-SY5Y cells become more resistant to JEV infection as they mature. This cell culture model is useful to elucidate the molecular mechanisms regulating the maturation state-specific resistance of neurons to JEV.</p>\",\"PeriodicalId\":49959,\"journal\":{\"name\":\"Journal of Veterinary Medical Science\",\"volume\":\" \",\"pages\":\"426-430\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964864/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Medical Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1292/jvms.24-0405\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/2/24 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medical Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1292/jvms.24-0405","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/24 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
In vitro susceptibility of differentiated SH-SY5Y human neuroblastoma cells to herpes simplex virus type 1 and Japanese encephalitis virus infection.
This study aimed to investigate the effects of cell differentiation status and neurotropic viral infectivity on the susceptibility of undifferentiated and differentiated SH-SY5Y cells to herpes simplex virus type 1 (HSV-1) and Japanese encephalitis virus (JEV) infections. Notably, undifferentiated SH-SY5Y cells were susceptible to both HSV-1 and JEV infections. However, differentiated cells exhibiting a phenotypically mature neuron-like morphology and markedly increased neuronal marker levels showed low susceptibility to JEV infection. Overall, these findings suggest that SH-SY5Y cells become more resistant to JEV infection as they mature. This cell culture model is useful to elucidate the molecular mechanisms regulating the maturation state-specific resistance of neurons to JEV.
期刊介绍：
JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
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