Lam Nguyen-Ngoc, Dang-Minh Luat, H Doan-Nhu, H M Pham, B Krock, N D Huynh-Thi, L V Tran-Thi, M H Tran-Thi, Anh H Pham, V Nguyen-Tam, T T Nhan-Luu, H H Do
{"title":"热带水域五种潜在有害甲藻（甲藻科，Gonyaulacales, Pyrocystaceae）： A. affine， A. fraterculus， A. leei， A. pseudogonyaulax和A. tamiyavanichii的系统发育和生物学特征。","authors":"Lam Nguyen-Ngoc, Dang-Minh Luat, H Doan-Nhu, H M Pham, B Krock, N D Huynh-Thi, L V Tran-Thi, M H Tran-Thi, Anh H Pham, V Nguyen-Tam, T T Nhan-Luu, H H Do","doi":"10.3390/toxins17020081","DOIUrl":null,"url":null,"abstract":"<p><p>Five species of <i>Alexandrium</i> (<i>A. affine</i>, <i>A</i>. <i>fraterculus</i>, <i>A</i>. <i>leei</i>, <i>A</i>. <i>pseudogonyaulax,</i> and <i>A</i>. <i>tamiyavanichii</i>) are commonly found in Vietnamese waters. They were distinguished based on their apical pore complex (A.P.C), precingular first plate (1'), ventral pore (Vp), and sulcal platelets. A genetic analysis was conducted using nuclear rDNA sequences of ITS and LSU (D1-D3, D8-D10). The growth rates of <i>A</i>. <i>fraterculus</i>, <i>A</i>. <i>leei</i>, <i>A</i>. <i>tamiyavanichii</i>, and <i>A</i>. <i>pseudogonyaulax</i> were quite similar. Specifically, these four species had the highest growth rates at two temperature levels of 24 °C and 27 °C, at salinities ranging from 25 psu to 35 psu. Furthermore, these species were able to adapt to a low salinity of 20 psu at temperatures from 18 °C to 27 °C. No Paralytic Shellfish Toxins (PSTs) were found in the two <i>Alexandrium affine</i> strains, VINVN01-1 and VINVN01-2. The detection limit for PSTs ranged from 0.45 to 15.5 fg cell<sup>-1</sup>, depending on the molecular response and available biomass.</p>","PeriodicalId":23119,"journal":{"name":"Toxins","volume":"17 2","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11862000/pdf/","citationCount":"0","resultStr":"{\"title\":\"Phylogenetic and Autecology Characteristics of Five Potentially Harmful Dinoflagellate <i>Alexandrium</i> Species (Dinophyceae, Gonyaulacales, Pyrocystaceae) in Tropical Waters: <i>A. affine</i>, <i>A. fraterculus</i>, <i>A. leei</i>, <i>A. pseudogonyaulax</i>, and <i>A. tamiyavanichii</i>.\",\"authors\":\"Lam Nguyen-Ngoc, Dang-Minh Luat, H Doan-Nhu, H M Pham, B Krock, N D Huynh-Thi, L V Tran-Thi, M H Tran-Thi, Anh H Pham, V Nguyen-Tam, T T Nhan-Luu, H H Do\",\"doi\":\"10.3390/toxins17020081\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Five species of <i>Alexandrium</i> (<i>A. affine</i>, <i>A</i>. <i>fraterculus</i>, <i>A</i>. <i>leei</i>, <i>A</i>. <i>pseudogonyaulax,</i> and <i>A</i>. <i>tamiyavanichii</i>) are commonly found in Vietnamese waters. They were distinguished based on their apical pore complex (A.P.C), precingular first plate (1'), ventral pore (Vp), and sulcal platelets. A genetic analysis was conducted using nuclear rDNA sequences of ITS and LSU (D1-D3, D8-D10). The growth rates of <i>A</i>. <i>fraterculus</i>, <i>A</i>. <i>leei</i>, <i>A</i>. <i>tamiyavanichii</i>, and <i>A</i>. <i>pseudogonyaulax</i> were quite similar. Specifically, these four species had the highest growth rates at two temperature levels of 24 °C and 27 °C, at salinities ranging from 25 psu to 35 psu. Furthermore, these species were able to adapt to a low salinity of 20 psu at temperatures from 18 °C to 27 °C. No Paralytic Shellfish Toxins (PSTs) were found in the two <i>Alexandrium affine</i> strains, VINVN01-1 and VINVN01-2. The detection limit for PSTs ranged from 0.45 to 15.5 fg cell<sup>-1</sup>, depending on the molecular response and available biomass.</p>\",\"PeriodicalId\":23119,\"journal\":{\"name\":\"Toxins\",\"volume\":\"17 2\",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-02-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11862000/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Toxins\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3390/toxins17020081\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxins","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/toxins17020081","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
越南水域常见五种亚历山大藻（A. affine， A. fraterculus， A. leei， A. pseudogonyaulax和A. tamiyavanichii）。根据尖孔复合体（apc）、鞘前第一板（1'）、腹侧孔（Vp）和沟侧血小板来区分。利用ITS和LSU的核rDNA序列（D1-D3, D8-D10）进行遗传分析。三种菌株的生长速率基本一致，分别为：异卵拟南芥、李氏拟南芥、tamiyavanici和pseudogonyaulax。具体来说，这四个物种在24°C和27°C两个温度水平下，在25至35 psu的盐度范围内，生长速度最高。此外，这些物种能够在18°C至27°C的温度下适应20 psu的低盐度。2株亚历山大菌仿射菌株VINVN01-1和VINVN01-2未检出麻痹性贝类毒素（PSTs）。PSTs的检出限范围为0.45至15.5 fg cell-1，取决于分子响应和可利用生物量。
Phylogenetic and Autecology Characteristics of Five Potentially Harmful Dinoflagellate Alexandrium Species (Dinophyceae, Gonyaulacales, Pyrocystaceae) in Tropical Waters: A. affine, A. fraterculus, A. leei, A. pseudogonyaulax, and A. tamiyavanichii.
Five species of Alexandrium (A. affine, A. fraterculus, A. leei, A. pseudogonyaulax, and A. tamiyavanichii) are commonly found in Vietnamese waters. They were distinguished based on their apical pore complex (A.P.C), precingular first plate (1'), ventral pore (Vp), and sulcal platelets. A genetic analysis was conducted using nuclear rDNA sequences of ITS and LSU (D1-D3, D8-D10). The growth rates of A. fraterculus, A. leei, A. tamiyavanichii, and A. pseudogonyaulax were quite similar. Specifically, these four species had the highest growth rates at two temperature levels of 24 °C and 27 °C, at salinities ranging from 25 psu to 35 psu. Furthermore, these species were able to adapt to a low salinity of 20 psu at temperatures from 18 °C to 27 °C. No Paralytic Shellfish Toxins (PSTs) were found in the two Alexandrium affine strains, VINVN01-1 and VINVN01-2. The detection limit for PSTs ranged from 0.45 to 15.5 fg cell-1, depending on the molecular response and available biomass.
期刊介绍：
Toxins (ISSN 2072-6651) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to toxins and toxinology. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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