Annalisa Buonanno, Marianna Imparato, Angela Maione, Federica Carraturo, Emilia Galdiero, Marco Guida, Elisabetta de Alteriis
{"title":"The Biotherapeutic Potential of a Novel Probiotic <i>Kluyveromyces marxianus</i> Isolated from a Sourdough Starter Against Vaginal <i>Candida albicans</i> Strains.","authors":"Annalisa Buonanno, Marianna Imparato, Angela Maione, Federica Carraturo, Emilia Galdiero, Marco Guida, Elisabetta de Alteriis","doi":"10.3390/jof11020147","DOIUrl":null,"url":null,"abstract":"<p><p>There is an increasing interest in yeasts isolated from natural sources to be used as probiotics. <i>Saccharomyces</i>-based probiotics have been proposed as a valid alternative to the conventional drug therapy for the prevention and treatment of vulvovaginal candidiasis, also considering the resistance of some <i>Candida</i> strains to many antifungals. Here, we isolated from an artisanal sourdough a new yeast strain which was identified as <i>Kluyveromyces marxianus</i> and assessed its probiotic and safety properties, which resulted in comparable properties to all those exhibited by the commercial probiotic <i>Saccharomyces boulardii</i>. Then, we checked the antagonistic activity of the new isolate against some clinical fluconazole resistant <i>C. albicans</i> strains, showing its ability to inhibit filamentation, biofilm formation, and the adhesion of <i>C. albicans</i> to vaginal epithelial A-431 cells. Also, <i>K. marxianus</i> reduced the cell damage provoked by <i>C. albicans</i> and the expression of <i>SAP2</i> and <i>SAP6</i> genes. On the whole, our results enlarge the spectrum of the beneficial properties of the food-grade yeast <i>K. marxianus</i> showing for the first time its biotherapeutic potential against <i>C. albicans</i>.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856499/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fungi","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/jof11020147","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
The Biotherapeutic Potential of a Novel Probiotic Kluyveromyces marxianus Isolated from a Sourdough Starter Against Vaginal Candida albicans Strains.
There is an increasing interest in yeasts isolated from natural sources to be used as probiotics. Saccharomyces-based probiotics have been proposed as a valid alternative to the conventional drug therapy for the prevention and treatment of vulvovaginal candidiasis, also considering the resistance of some Candida strains to many antifungals. Here, we isolated from an artisanal sourdough a new yeast strain which was identified as Kluyveromyces marxianus and assessed its probiotic and safety properties, which resulted in comparable properties to all those exhibited by the commercial probiotic Saccharomyces boulardii. Then, we checked the antagonistic activity of the new isolate against some clinical fluconazole resistant C. albicans strains, showing its ability to inhibit filamentation, biofilm formation, and the adhesion of C. albicans to vaginal epithelial A-431 cells. Also, K. marxianus reduced the cell damage provoked by C. albicans and the expression of SAP2 and SAP6 genes. On the whole, our results enlarge the spectrum of the beneficial properties of the food-grade yeast K. marxianus showing for the first time its biotherapeutic potential against C. albicans.
期刊介绍:
Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.