IF 4.2 2区 生物学 Q2 MICROBIOLOGY
Annalisa Buonanno, Marianna Imparato, Angela Maione, Federica Carraturo, Emilia Galdiero, Marco Guida, Elisabetta de Alteriis
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引用次数: 0

摘要

人们对从天然资源中分离出来用作益生菌的酵母菌越来越感兴趣。考虑到一些念珠菌菌株对许多抗真菌药的耐药性,人们提出了以酵母菌为基础的益生菌,作为预防和治疗外阴阴道念珠菌病的传统药物疗法的有效替代品。在这里,我们从手工制作的酸面团中分离出了一种新的酵母菌株,并对其益生菌特性和安全性进行了评估,其结果与商业益生菌布拉氏酵母菌(Saccharomyces boulardii)的所有特性相当。然后,我们检测了新分离菌株对一些临床氟康唑耐药白僵菌菌株的拮抗活性,结果表明它能够抑制白僵菌菌丝的形成、生物膜的形成以及白僵菌对阴道上皮 A-431 细胞的粘附。此外,K. marxianus 还能减少白僵菌对细胞的损伤,降低 SAP2 和 SAP6 基因的表达。总之,我们的研究结果扩大了食品级酵母菌 K. marxianus 的有益特性范围,首次显示了它对白僵菌的生物治疗潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Biotherapeutic Potential of a Novel Probiotic Kluyveromyces marxianus Isolated from a Sourdough Starter Against Vaginal Candida albicans Strains.

There is an increasing interest in yeasts isolated from natural sources to be used as probiotics. Saccharomyces-based probiotics have been proposed as a valid alternative to the conventional drug therapy for the prevention and treatment of vulvovaginal candidiasis, also considering the resistance of some Candida strains to many antifungals. Here, we isolated from an artisanal sourdough a new yeast strain which was identified as Kluyveromyces marxianus and assessed its probiotic and safety properties, which resulted in comparable properties to all those exhibited by the commercial probiotic Saccharomyces boulardii. Then, we checked the antagonistic activity of the new isolate against some clinical fluconazole resistant C. albicans strains, showing its ability to inhibit filamentation, biofilm formation, and the adhesion of C. albicans to vaginal epithelial A-431 cells. Also, K. marxianus reduced the cell damage provoked by C. albicans and the expression of SAP2 and SAP6 genes. On the whole, our results enlarge the spectrum of the beneficial properties of the food-grade yeast K. marxianus showing for the first time its biotherapeutic potential against C. albicans.

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来源期刊
Journal of Fungi
Journal of Fungi Medicine-Microbiology (medical)
CiteScore
6.70
自引率
14.90%
发文量
1151
审稿时长
11 weeks
期刊介绍: Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.
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