Siyi Lin, Huangjie Fu, Hanxiao Wang, Yingying Xu, Yu Zhao, Shiyu Du, Jiale Wei, Ping Qiu, Senlin Shi, Changyu Li, Thomas Efferth, Chunlan Hong
{"title":"通过肠道-生殖系神经信号传导改善秀丽隐杆线虫肠道胀气依赖性回避行为","authors":"Siyi Lin, Huangjie Fu, Hanxiao Wang, Yingying Xu, Yu Zhao, Shiyu Du, Jiale Wei, Ping Qiu, Senlin Shi, Changyu Li, Thomas Efferth, Chunlan Hong","doi":"10.1111/cns.70291","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Purpose</h3>\n \n <p>The aim of this study was to investigate the protective effect of <i>Free and Easy Wanderer</i> (FAEW) on the avoidance behavior induced by feeding Heat-Killed <i>Escherichia coli</i>, and to elucidate the underlying mechanisms.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Initially, the effects of FAEW on avoidance behavior, survival, neuroendocrine signaling gene expression, and intestinal bloating were examined. The impact of FAEW on gut-germline-neural signaling was assessed by monitoring H4K8ac expression and the avoidance behavior of <i>par-5</i> RNAi animals and <i>glp-1(e2141)</i> mutants. RNA-sequencing was conducted to analyze potential signaling pathways. Finally, avoidance behavior was examined using <i>daf-16(mu86)</i> mutants and the rescued animals.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>FAEW delayed avoidance behavior. FAEW significantly downregulated gene expression in the neuroendocrine signaling pathway and alleviated intestinal bloating of <i>C. elegans</i>. The levels of H4K8ac and <i>par-5</i> in the germline decreased significantly with FAEW's treatment, and FAEW failed to affect the avoidance behavior of <i>par-5</i> RNAi animals and <i>glp-1(e2141)</i> mutants. FAEW's effect on avoidance behavior diminished in <i>daf-16(mu86)</i> mutants but was restored in <i>daf-16</i> rescued animals. FAEW has been observed to restore <i>daf-16</i> levels.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>FAEW protects against avoidance behavior of <i>C. elegans</i> through downregulating H4K8ac protein expression and activating DAF-16. This study provides crucial experimental evidence supporting FAEW as a promising candidate for protecting against avoidance behavior associated with PTSD.</p>\n </section>\n </div>","PeriodicalId":154,"journal":{"name":"CNS Neuroscience & Therapeutics","volume":"31 2","pages":""},"PeriodicalIF":5.0000,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cns.70291","citationCount":"0","resultStr":"{\"title\":\"Free and Easy Wanderer Ameliorates Intestinal Bloating-Dependent Avoidance Behavior of Caenorhabditis elegans Through Gut-Germline-Neural Signaling\",\"authors\":\"Siyi Lin, Huangjie Fu, Hanxiao Wang, Yingying Xu, Yu Zhao, Shiyu Du, Jiale Wei, Ping Qiu, Senlin Shi, Changyu Li, Thomas Efferth, Chunlan Hong\",\"doi\":\"10.1111/cns.70291\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Purpose</h3>\\n \\n <p>The aim of this study was to investigate the protective effect of <i>Free and Easy Wanderer</i> (FAEW) on the avoidance behavior induced by feeding Heat-Killed <i>Escherichia coli</i>, and to elucidate the underlying mechanisms.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Initially, the effects of FAEW on avoidance behavior, survival, neuroendocrine signaling gene expression, and intestinal bloating were examined. The impact of FAEW on gut-germline-neural signaling was assessed by monitoring H4K8ac expression and the avoidance behavior of <i>par-5</i> RNAi animals and <i>glp-1(e2141)</i> mutants. RNA-sequencing was conducted to analyze potential signaling pathways. Finally, avoidance behavior was examined using <i>daf-16(mu86)</i> mutants and the rescued animals.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>FAEW delayed avoidance behavior. FAEW significantly downregulated gene expression in the neuroendocrine signaling pathway and alleviated intestinal bloating of <i>C. elegans</i>. The levels of H4K8ac and <i>par-5</i> in the germline decreased significantly with FAEW's treatment, and FAEW failed to affect the avoidance behavior of <i>par-5</i> RNAi animals and <i>glp-1(e2141)</i> mutants. FAEW's effect on avoidance behavior diminished in <i>daf-16(mu86)</i> mutants but was restored in <i>daf-16</i> rescued animals. FAEW has been observed to restore <i>daf-16</i> levels.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>FAEW protects against avoidance behavior of <i>C. elegans</i> through downregulating H4K8ac protein expression and activating DAF-16. 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Free and Easy Wanderer Ameliorates Intestinal Bloating-Dependent Avoidance Behavior of Caenorhabditis elegans Through Gut-Germline-Neural Signaling
Purpose
The aim of this study was to investigate the protective effect of Free and Easy Wanderer (FAEW) on the avoidance behavior induced by feeding Heat-Killed Escherichia coli, and to elucidate the underlying mechanisms.
Methods
Initially, the effects of FAEW on avoidance behavior, survival, neuroendocrine signaling gene expression, and intestinal bloating were examined. The impact of FAEW on gut-germline-neural signaling was assessed by monitoring H4K8ac expression and the avoidance behavior of par-5 RNAi animals and glp-1(e2141) mutants. RNA-sequencing was conducted to analyze potential signaling pathways. Finally, avoidance behavior was examined using daf-16(mu86) mutants and the rescued animals.
Results
FAEW delayed avoidance behavior. FAEW significantly downregulated gene expression in the neuroendocrine signaling pathway and alleviated intestinal bloating of C. elegans. The levels of H4K8ac and par-5 in the germline decreased significantly with FAEW's treatment, and FAEW failed to affect the avoidance behavior of par-5 RNAi animals and glp-1(e2141) mutants. FAEW's effect on avoidance behavior diminished in daf-16(mu86) mutants but was restored in daf-16 rescued animals. FAEW has been observed to restore daf-16 levels.
Conclusion
FAEW protects against avoidance behavior of C. elegans through downregulating H4K8ac protein expression and activating DAF-16. This study provides crucial experimental evidence supporting FAEW as a promising candidate for protecting against avoidance behavior associated with PTSD.
期刊介绍：
CNS Neuroscience & Therapeutics provides a medium for rapid publication of original clinical, experimental, and translational research papers, timely reviews and reports of novel findings of therapeutic relevance to the central nervous system, as well as papers related to clinical pharmacology, drug development and novel methodologies for drug evaluation. The journal focuses on neurological and psychiatric diseases such as stroke, Parkinson’s disease, Alzheimer’s disease, depression, schizophrenia, epilepsy, and drug abuse.
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