卫生保健相关牙髓感染中恢复的Cutibacterium sp .的全基因组测序分析

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology Pub Date : 2025-02-21 DOI:10.1016/j.archoralbio.2025.106207

Mónica Álvarez-Muñoz , Christian Jerez-Olate , Andrés Opazo-Capurro , Raúl Alcántara-Dufeu , Helia Bello-Toledo , Gerardo González-Rocha , Gabriela Sánchez-Sanhueza

{"title":"卫生保健相关牙髓感染中恢复的Cutibacterium sp .的全基因组测序分析","authors":"Mónica Álvarez-Muñoz , Christian Jerez-Olate , Andrés Opazo-Capurro , Raúl Alcántara-Dufeu , Helia Bello-Toledo , Gerardo González-Rocha , Gabriela Sánchez-Sanhueza","doi":"10.1016/j.archoralbio.2025.106207","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div><em>Cutibacterium acnes</em> (<em>C. acnes</em>) has been detected in culture and molecular studies of endodontic microbiota, indicating its potential as a neglected pathogen in endodontic infections. This study aimed to conduct a complete genomic analysis of three <em>Cutibacterium</em> spp. isolates obtained from persistent endodontic infections in patients from Chile.</div></div><div><h3>Design</h3><div>Whole genome sequencing and comprehensive genomic analysis using diverse bioinformatics platforms and databases were carried out.</div></div><div><h3>Results</h3><div><em>In-silico</em> analysis confirmed that strains A1 and E3 correspond to <em>C. acnes</em> species, while strain B1 corresponds to <em>C. namnetense</em>. Additionally, <em>C. acnes</em> strain A1 belongs to the phylotype IA<sub>1</sub>, sequence type (ST) 4 and belongs to the clonal complex (CC) 4, whereas <em>C. acnes</em> strain E3 belongs to a novel ST. No antibiotic resistance, however, a variable distribution and identity of the VF was found.</div></div><div><h3>Conclusions</h3><div>Detailed analysis of genes associated with virulence factors shed light on the intrinsic pathogenic capacity of these strains, which may indicate differential adaptation to their specific niches or diversity in pathogenicity within the species.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106207"},"PeriodicalIF":2.2000,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Whole-genome sequencing analysis of Cutibacterium spp. recovered from health care-associated endodontic infections\",\"authors\":\"Mónica Álvarez-Muñoz , Christian Jerez-Olate , Andrés Opazo-Capurro , Raúl Alcántara-Dufeu , Helia Bello-Toledo , Gerardo González-Rocha , Gabriela Sánchez-Sanhueza\",\"doi\":\"10.1016/j.archoralbio.2025.106207\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div><em>Cutibacterium acnes</em> (<em>C. acnes</em>) has been detected in culture and molecular studies of endodontic microbiota, indicating its potential as a neglected pathogen in endodontic infections. This study aimed to conduct a complete genomic analysis of three <em>Cutibacterium</em> spp. isolates obtained from persistent endodontic infections in patients from Chile.</div></div><div><h3>Design</h3><div>Whole genome sequencing and comprehensive genomic analysis using diverse bioinformatics platforms and databases were carried out.</div></div><div><h3>Results</h3><div><em>In-silico</em> analysis confirmed that strains A1 and E3 correspond to <em>C. acnes</em> species, while strain B1 corresponds to <em>C. namnetense</em>. Additionally, <em>C. acnes</em> strain A1 belongs to the phylotype IA<sub>1</sub>, sequence type (ST) 4 and belongs to the clonal complex (CC) 4, whereas <em>C. acnes</em> strain E3 belongs to a novel ST. No antibiotic resistance, however, a variable distribution and identity of the VF was found.</div></div><div><h3>Conclusions</h3><div>Detailed analysis of genes associated with virulence factors shed light on the intrinsic pathogenic capacity of these strains, which may indicate differential adaptation to their specific niches or diversity in pathogenicity within the species.</div></div>\",\"PeriodicalId\":8288,\"journal\":{\"name\":\"Archives of oral biology\",\"volume\":\"173 \",\"pages\":\"Article 106207\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-02-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of oral biology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0003996925000354\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of oral biology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003996925000354","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

摘要

目的在牙髓微生物群的培养和分子研究中发现了痤疮芽胞杆菌（C. acnes），提示其在牙髓感染中可能是一种被忽视的病原体。本研究旨在对智利患者持续性牙髓感染中分离的三株Cutibacterium spp.进行完整的基因组分析。设计利用多种生物信息学平台和数据库进行全基因组测序和综合基因组分析。结果菌株A1和E3与C. acnes属对应，菌株B1与C. namnetense属对应。此外，痤疮C. A1菌株属于IA1型，序列型（ST） 4，属于克隆复合体（CC） 4，而痤疮C. E3菌株属于一种新型ST.，但没有发现抗生素耐药，但发现VF分布和身份不同。结论对毒力因子相关基因的详细分析揭示了这些菌株的内在致病能力，这可能表明它们对特定生态位的不同适应或物种内致病性的多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Whole-genome sequencing analysis of Cutibacterium spp. recovered from health care-associated endodontic infections

Objective

Cutibacterium acnes (C. acnes) has been detected in culture and molecular studies of endodontic microbiota, indicating its potential as a neglected pathogen in endodontic infections. This study aimed to conduct a complete genomic analysis of three Cutibacterium spp. isolates obtained from persistent endodontic infections in patients from Chile.

Design

Whole genome sequencing and comprehensive genomic analysis using diverse bioinformatics platforms and databases were carried out.

Results

In-silico analysis confirmed that strains A1 and E3 correspond to C. acnes species, while strain B1 corresponds to C. namnetense. Additionally, C. acnes strain A1 belongs to the phylotype IA₁, sequence type (ST) 4 and belongs to the clonal complex (CC) 4, whereas C. acnes strain E3 belongs to a novel ST. No antibiotic resistance, however, a variable distribution and identity of the VF was found.

Conclusions

Detailed analysis of genes associated with virulence factors shed light on the intrinsic pathogenic capacity of these strains, which may indicate differential adaptation to their specific niches or diversity in pathogenicity within the species.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Archives of oral biology 医学-牙科与口腔外科

CiteScore

5.10

自引率

3.30%

发文量

177

审稿时长

26 days

期刊介绍： Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry