关掉暖气

IF 12.9 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nature chemical biology Pub Date : 2025-02-25 DOI:10.1038/s41589-025-01855-x

Grant Miura

引用次数: 0

摘要

利用光反应蛋白可以实现细胞信号和行为的时空调节。然而，由于光不能穿透组织超过几毫米，在体内的应用仍然有限。虽然热敏蛋白已被提出作为一种替代方法，但很少有工具是可调的，可逆的和模块化的。BcLOV4是一种光遗传蛋白，可调节膜在光和温度下的定位。为了产生只对温度变化有反应的BcLOV4变体，Benman等人发现了一种具有单点突变的蛋白质，他们将其命名为Melt。熔体在25℃时表现出膜的聚集和聚集，但当温度升高到37℃时，膜的局部化程度降低。在温度范围内对熔体的广泛表征表明，在27°C时观察到最大的膜结合，在32°C时消失。熔体与信号效应器的端到端融合在27°C时产生活性，而当转移到32°C时下游信号传导减少。接下来，该团队使用额外的点突变和融合到多基域，生成了具有改变热开关点的Melt变体。特别是，Melt-37变体可以在37°C及以上诱导变化，使其成为体内应用的理想选择。使用热电冷却装置将Melt-37与caspase-1效应域融合可导致小鼠肺癌异种移植物局部细胞死亡，但不会改变应激颗粒或增殖。总的来说，Melt提供了一个有用的工具，可以在指定的热范围内实现细胞的快速变化。原始参考文献：Nat. Methods https://doi.org/10.1038/s41592-024-02572-4 （2025）

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来源期刊

Nature chemical biology 生物-生化与分子生物学

CiteScore

23.90

自引率

1.40%

发文量

238

审稿时长

12 months

期刊介绍： Nature Chemical Biology stands as an esteemed international monthly journal, offering a prominent platform for the chemical biology community to showcase top-tier original research and commentary. Operating at the crossroads of chemistry, biology, and related disciplines, chemical biology utilizes scientific ideas and approaches to comprehend and manipulate biological systems with molecular precision. The journal embraces contributions from the growing community of chemical biologists, encompassing insights from chemists applying principles and tools to biological inquiries and biologists striving to comprehend and control molecular-level biological processes. We prioritize studies unveiling significant conceptual or practical advancements in areas where chemistry and biology intersect, emphasizing basic research, especially those reporting novel chemical or biological tools and offering profound molecular-level insights into underlying biological mechanisms. Nature Chemical Biology also welcomes manuscripts describing applied molecular studies at the chemistry-biology interface due to the broad utility of chemical biology approaches in manipulating or engineering biological systems. Irrespective of scientific focus, we actively seek submissions that creatively blend chemistry and biology, particularly those providing substantial conceptual or methodological breakthroughs with the potential to open innovative research avenues. The journal maintains a robust and impartial review process, emphasizing thorough chemical and biological characterization.