Gabriela Manrique, Jérémie Gottraux, Simon Matoori
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Mechanistic Insights into HRP-Catalyzed Oxidation of the Near-Infrared Fluorescent Dye Sulfo-cyanine 7.
Horseradish peroxidase (HRP) is a widely used enzyme that oxidizes a range of substrates in the presence of its co-substrate hydrogen peroxide (H2O2). In the past, we identified sulfo-cyanine 7 (S7) as a novel near-infrared substrate of HRP, which loses its fluorescence upon enzymatic oxidation in a hydrogen peroxide-dependent manner. However, the catalytic mechanism and the chemical structure of the oxidation products were not well understood. In this study, we investigate the catalytic mechanism of S7 oxidation by HRP and identify two radical products formed during the reaction. Our results point to the formation of non-near-infrared fluorescent species, and the regeneration of S7. These results strongly point to a disproportionation reaction that was described for other HRP substrates, namely the structurally related ABTS (2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)).
期刊介绍：
ACS Measurement Science Au is an open access journal that publishes experimental computational or theoretical research in all areas of chemical measurement science. Short letters comprehensive articles reviews and perspectives are welcome on topics that report on any phase of analytical operations including sampling measurement and data analysis. This includes:Chemical Reactions and SelectivityChemometrics and Data ProcessingElectrochemistryElemental and Molecular CharacterizationImagingInstrumentationMass SpectrometryMicroscale and Nanoscale systemsOmics (Genomics Proteomics Metabonomics Metabolomics and Bioinformatics)Sensors and Sensing (Biosensors Chemical Sensors Gas Sensors Intracellular Sensors Single-Molecule Sensors Cell Chips Arrays Microfluidic Devices)SeparationsSpectroscopySurface analysisPapers dealing with established methods need to offer a significantly improved original application of the method.
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