miR-379-5p Inhibited the Proliferation of Acute Myeloid Leukemia Cells Through Negative Regulation of YBX1

Objective: Acute myeloid leukemia (AML) highly lethal hematological malignancy that is difficult to treat. This study aimed to clarify the molecular mechanisms of miR-379-5p in AML progression.

Materials and methods: Quantitative real-time polymerase chain reaction was utilized to evaluate miR-379-5p expression levels in AML patients and a control group. A receiver operating characteristic curve was created to assess the clinical predictive value of miR-379-5p in AML, while cell experiments used the CCK-8 assay, flow cytometry, and transwell chambers. Potential target genes of miR-379-5p were predicted by employing online bioinformatics tools, followed by validation using a dual luciferase reporter assay.

Results: miR-379-5p expression was significantly decreased in AML patients and had clinical predictive value for the disease. In AML cell lines, miR-379-5p was downregulated; conversely, the upregulation of miR-379-5p inhibited proliferation, migration, and invasion while promoting apoptosis. Notably, YBX1 was a potential target gene of miR-379-5p and its upregulation reduced the effects of miR-379-5p on AML cell behavior.

Conclusion: miR-379-5p has potential as a biomarker for AML by regulating cell proliferation and apoptosis through the targeting of YBX1.