蓝光下水稻颖果类黄酮积累机制的多组学分析。

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Ping Zhang, Yongsheng Tang, Juxiang Zhang, Junna Liu, Li Li, Hanxue Li, Liubin Huang, Guofei Jiang, Xuqin Wang, Lingyuan Zhang, Yutao Bai, Peng Qin
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引用次数: 0

摘要

关键信息:蓝光影响MYB基因家族,导致不同发育阶段水稻颖果中不同黄酮类化合物的积累不同,在初始阶段观察到较高的浓度。蓝光对植物类黄酮的调控作用已被广泛报道;然而,其对水稻颖果形态发育的影响尚未见报道。通过转录组、蛋白质组和代谢物分析,结合加权基因共表达网络分析(WGCNA),全面研究了蓝光照射下水稻颖果中黄酮类化合物在不同发育阶段的积累情况。此外,还鉴定了4个MYB家族转录因子(TFs),这些转录因子对类黄酮生物合成相关的结构基因有显著影响。结果表明,黄酮类化合物的积累主要发生在颖果发育的早期阶段。PAL、4CL、CHS、CHI、F3H、FLS等关键结构基因在蓝光照射下基因和蛋白表达均上调。此外,WGCNA分析还发现了几个可能影响这些基因的tf,包括Os08t0144000-01和Os01t0695900-01,以及蛋白Q7F3D6、Q2QM89、A0A0P0W9C3和Q6ZDM0,它们都属于MYB家族。该研究增加了我们对蓝光照射下水稻颖果类黄酮积累的认识。它还为蓝光诱导的次生代谢物的合成建立了框架,从而为提高园艺植物的质量创造了更多的机会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multi-omics analysis of the accumulation mechanism of flavonoids in rice caryopsis under blue light.

Key message: Blue light influences the MYB gene family, resulting in varying accumulations of different flavonoids in rice caryopsis at distinct developmental stages, with a higher concentration observed in the initial stage. The regulatory effect of blue light on plant flavonoids has been extensively documented; however, its influence on the development of rice caryopsis morphology remains unreported. Through the analysis of transcriptomes, proteomes, and metabolites, combined with Weighted Gene Co-expression Network Analysis (WGCNA), the accumulation of flavonoids in rice caryopsis under blue light at various developmental stages was thoroughly examined. Furthermore, four MYB family transcription factors (TFs) that significantly influence the structural genes involved in flavonoid biosynthesis were identified. The results indicate that the accumulation of flavonoids primarily occurs during the early stages of caryopsis development. Key structural genes, including PAL, 4CL, CHS, CHI, F3H, and FLS, are upregulated in both gene and protein expression when exposed to blue light. Moreover, the WGCNA analysis identified several TFs that may influence these genes, including Os08t0144000-01 and Os01t0695900-01, as well as the proteins Q7F3D6, Q2QM89, A0A0P0W9C3, and Q6ZDM0, all of which belong to the MYB family. The research has enhanced our understanding of flavonoid accumulation in rice caryopsis when exposed to blue light. It also establishes a framework for the synthesis of secondary metabolites induced by blue light, thereby creating more opportunities to enhance the quality of horticultural plants.

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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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