Fariba Ashrafi, Monika Astasov-Frauenhoffer, Elizaveta Fasler-Kan, Sabrina Ruggiero, Roland Steiner, Laurent Marot, Fabien Sanchez, Sebastian Kühl, Michael M Bornstein, Khaled Mukaddam
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引用次数: 0
摘要
目的：本研究的目的是评估纳米结构牙种植体表面对牙龈卟啉单胞菌的抗菌效果，牙龈卟啉单胞菌是种植体周围炎进展中的关键细菌。此外，评估改良种植体表面对人牙龈成纤维细胞（HGnFs）活力的可能影响。方法与材料：如前期研究所述，采用氦离子溅射法制备高度为1000 nm （Ti1000）的纳米尖刺钛表面。光滑加工（Ti machined）和SLA钛盘（TiSLA）作为对照。通过常规培养和扫描电镜观察样品对牙龈卟啉单胞菌的抑菌特性。使用MTT法评估hgnf的活力。结果：一般来说，纳米结构的Ti1000表面的细菌数量比两个对照组（Ti1000 vs TiSLA -36%， Ti1000 vs Ti加工-37%）少，但没有统计学意义。此外，纳米表面不影响hgnf的活力。结论：改良钛种植体表面的纳米尖不影响成纤维细胞的活力和增殖特性。优化纳米尖的间距和高度参数可以进一步提高这种独特的再修饰方法的抗菌效果。
Adhesion of Porphyromonas gingivalis and growth of human gingival fibroblasts on modified titanium dental implant surfaces exhibiting 1000-nm spikes.
Objectives: The aim of this study was to assess the antibacterial efficacy of nanostructured dental implant surfaces against Porphyromonas gingivalis, a crucial bacterium in the progression of peri-implantitis. Additionally, to evaluate the possible effect of the modified implant surface on the viability of human gingival fibroblasts (HGnFs).
Method and materials: As detailed in prior research, helium ions sputtering was used to produce nanospiked titanium surfaces with a height of 1000 nm (Ti1000). Smooth machined (Ti machined) and SLA titanium disks (TiSLA) served as controls. The antibacterial characteristics of the samples against P. gingivalis were evaluated by conventional culturing and SEM. The vitality of HGnFs was assessed using the MTT assay.
Results: Generally, nanostructured Ti1000 surfaces exhibited less bacterial counts than the two controls (Ti1000 vs TiSLA -36% and Ti1000 vs Ti machined -37%.) without being statistically significant. Moreover, the nanosurface did not affect the vitality of HGnFs.
Conclusion: The nanospikes of the modified titanium implant surface did not hinder gingival fibroblasts' vitality or proliferation characteristics. Optimizing the spacing and height parameters of the nanospikes could further improve the antibacterial effects of this unique re-modification approach.
期刊介绍：
QI has a new contemporary design but continues its time-honored tradition of serving the needs of the general practitioner with clinically relevant articles that are scientifically based. Dr Eli Eliav and his editorial board are dedicated to practitioners worldwide through the presentation of high-level research, useful clinical procedures, and educational short case reports and clinical notes. Rigorous but timely manuscript review is the first order of business in their quest to publish a high-quality selection of articles in the multiple specialties and disciplines that encompass dentistry.
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