使用单克隆抗体D2-40 (Podoplanin)检测淋巴管侵袭作为乳腺癌患者腋窝淋巴结转移的临床预测指标。

Q2 Health Professions
Teresa Cistina Ferreira Gutman, Angela de Salles Rezende, Dyego Mondego Moraes, Consuelo Lozoya Lopez, Licínio Esmeraldo da Silva, Rafaela Elvira Rozza-de-Menezes, João Paulo Lima Daher, André Vallejo da Silva, Fabiana Resende Rodrigues, Vânia Gloria Silami Lopes
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引用次数: 0

摘要

乳腺癌是一个重大的全球健康问题,也是妇女中最常见的癌症。由于需要区分真正的入侵和伪影,识别血管入侵是具有挑战性的。本研究利用单克隆抗体D2-40作为预后指标探讨浸润性乳腺癌的淋巴栓塞。2009 - 2011年共纳入100例浸润性乳腺癌患者。组织微阵列技术(TMA)用于患者组织,从每位参与者的组织学数据构建三个石蜡块。分别用D2-40和CD34抗体进行免疫组化鉴定淋巴栓塞和血栓塞,并将结果与先前的发现进行比较。先前使用苏木精-伊红染色的报告发现,与我们使用D2-40的研究(56例)相比,较少的患者出现淋巴栓塞(34例)。淋巴栓塞与腋窝转移相关,优势比(OR)为3.50,95%可信区间(CI)为1.92-5.08,p值为0.001,而单独苏木精-伊红的OR = 1.42, 95% CI = 0.40-3.47, p值= 0.23。TMA与D2-40染色比单独苏木精-伊红染色检测到更多的淋巴栓塞。栓塞表达率越高,肿瘤侵袭性越强,预后越差,总生存期越短。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lymphangioinvasion detection using the monoclonal antibody D2-40 (Podoplanin)as a clinical predictor of axillary lymph node metastasis in breast cancer patients.

Breast is a major global health issue and the most common cancer in women. Identifying vascular invasion is challenging due to the need to distinguish true invasion from artifacts. This study explored lymphatic embolism in invasive breast carcinoma using the monoclonal antibody D2-40 as a prognostic indicator. A total of 100 patients with invasive breast carcinoma from 2009 to 2011 were included in the study. Tissue microarray technique (TMA) was used on patient tissue, constructing three paraffin blocks from each participant's histological data. Immunohistochemistry with D2-40 and CD34 antibodies was performed to identify lymphatic and blood emboli, respectively, and results were compared with previous findings. A prior report using hematoxylin-eosin staining found fewer patients with lymphatic emboli (34) compared to our study (56) using D2-40. Lymphatic emboli correlated with axillary metastases, with an odds ratio (OR) of 3.50, a 95% confidence interval (CI) of 1.92-5.08, and a p-value of 0.001, whereas hematoxylin-eosin alone showed OR = 1.42, 95% CI = 0.40-3.47, and p-value = 0.23. TMA with D2-40 staining detected more lymphatic emboli than hematoxylin-eosin staining alone. Higher embolic expression rates are linked to increased tumor aggressiveness, worse prognosis and shorter overall survival.

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来源期刊
CiteScore
3.50
自引率
0.00%
发文量
38
审稿时长
>12 weeks
期刊介绍: The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects of immunology. They include receptor assay, enzyme-linked immunosorbent assay (ELISA) in all of its embodiments, ligand-based assays, biological markers of ligand-receptor interaction, in vivo and in vitro diagnostic reagents and techniques, diagnosis of AIDS, point-of-care testing, clinical immunology, antibody isolation and purification, and others.
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