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IF 3.5 2区农林科学 Q2 INFECTIOUS DISEASES

Transboundary and Emerging Diseases Pub Date : 2025-02-23 DOI:10.1155/tbed/2883245

Wenchi Chang, Junyang Fang, Tianshu Zhai, Shuizhong Han, Wenqi Fan, Congshang Lei, Lizhen Wang, Xuefeng Qi, Qinghong Xue, Jingyu Wang

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引用次数: 0

摘要

结节性皮肤病（LSD）是威胁全球养牛业的一种重要传染病。最近，LSD 在亚洲频繁发生。在中国，山羊痘病毒减毒疫苗（GTPV）被广泛用于预防牛鳞屑性皮炎，但在免疫过的牛中仍有零星的鳞屑性皮炎病例发生。本研究旨在探讨分离的LSD病毒（LSDV）毒株在GTPV异源疫苗免疫压力下的遗传进化。我们从中国西北部陕西省一个使用GTPV减毒疫苗免疫的牛群中分离出一株新的LSDV，命名为LSDV/China/SX/2023，并进行了全基因组测序和遗传进化研究。该分离菌株与中国参考菌株存在多个开放阅读框（ORF）差异，其中ORF19编码的Kelch样蛋白的截短表达可能会影响其生长特性。系统进化分析表明，分离菌株与之前从中国和东南亚分离的菌株属于同一进化支系。RDP4和Simplot同时显示，该分支中的所有菌株都有相同的15个重组事件，其中有一个重组事件与GTPV有关。本研究介绍了 LSDV 在 GTPV 疫苗免疫压力下的最新基因进化，并讨论了被认为影响传播的病毒重组事件，这些事件对于 LSD 的控制和净化至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Genetic Evolutionary Analysis of Lumpy Skin Disease Virus Strain Under Immune Pressure Exerted by Heterologous Goat Poxvirus Vaccines

查看原文本刊更多论文

Genetic Evolutionary Analysis of Lumpy Skin Disease Virus Strain Under Immune Pressure Exerted by Heterologous Goat Poxvirus Vaccines

Lumpy skin disease (LSD) is an important infectious disease that threatens the global cattle industry. Recently, LSD has occurred frequently in Asia. The attenuated goat poxvirus (GTPV) vaccine is widely used to prevent LSD in cattle in China; however, sporadic cases of LSD still occur in immunized cattle. This study aimed to investigate the genetic evolution of isolated LSD virus (LSDV) strain under GTPV heterologous vaccine immune pressure. We isolated a new strain of LSDV, named LSDV/China/SX/2023, from a cattle herd immunized with a GTPV-attenuated vaccine in Shaanxi Province, Northwest China, and conducted whole-genome sequencing and genetic evolution studies. There are several open reading frames (ORFs) differences between the isolated strain and the Chinese reference strains, among which truncated expression of the Kelch-like protein encoded by ORF 19 may affect its growth characteristics. Phylogenetic analysis revealed that the isolated strain is in the same evolutionary clade as previous isolates from China and Southeast Asia. RDP4 and Simplot simultaneously showed that all strains in this branch had the same 15 recombination events, and there was one recombination event associated with the GTPV. This study presents the recent genetic evolution of LSDV under GTPV vaccine immune pressure and discusses the viral recombination events that are thought to influence transmission which are critical for the control and purification of LSD.

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来源期刊

Transboundary and Emerging Diseases 农林科学-传染病学

CiteScore

8.90

自引率

9.30%

发文量

350

审稿时长

1 months

期刊介绍： Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions): Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread. Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope. Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies. Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies). Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.