两种细菌胞外多糖对燕麦青贮微生物群落、发酵特性及有氧稳定性的影响

IF 5.7 2区 生物学
Wei Liu, Zhijun Wang, Lin Sun, Shuai Du, Gentu Ge, Yushan Jia
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引用次数: 0

摘要

本研究探讨了两种外多糖能否作为外源碳源提高燕麦青贮发酵品质,为其在青贮中的进一步应用提供理论基础。燕麦在抽穗期收获,经过一段时间的萎蔫,切成2-3厘米的长度用于青贮试验。处理方法如下:(1)对照组(CK),只给予无菌水;(2)加入葡聚糖(D)的一组;(3)添加levan (L)组。分别在3、7、14、30和60天(d)的不同时间间隔监测发酵过程。青贮60 d后,青贮5 d进行有氧暴露(AE)。EPS改变了青贮的发酵品质,改变了青贮的细菌群落组成,影响了样品组间的特征差异。同时,不同发酵时间EPS对碳水化合物代谢的调节作用不同。EPS处理提高了青贮饲料乳酸含量,降低了青贮饲料pH值。发酵60天后,乳酸杆菌的相对丰度也有所增加。葡聚糖和levan分别增加了Hafnia-Obesumbacterium和Sediminibacterium的相对丰度。在右旋糖酐的处理下,青贮饲料保留了更多的WSC含量,并取得了较高的好氧稳定性。通过比较细菌相关网络,很明显发酵时间改变了细菌间相关网络的组成。综上所述,EPS能有效提高燕麦青贮发酵品质,其中葡聚糖的正向效果最为显著。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of Two Bacterial Exopolysaccharides on Microbial Community, Fermentation Characteristics and Aerobic Stability in Oat Silage

Effects of Two Bacterial Exopolysaccharides on Microbial Community, Fermentation Characteristics and Aerobic Stability in Oat Silage

This study investigated whether two exopolysaccharides could serve as exogenous carbon sources to enhance fermentation quality in oat silage, providing a theoretical foundation for their future application in silage. The oats were harvested at the heading stage and, following a period of wilting, were chopped into 2–3 cm lengths for the ensiling experiment. The treatments applied were as follows: (1) a control group (CK), which received only sterile water; (2) a group with added dextran (D); and (3) a group with added levan (L). The fermentation process was monitored at various intervals: 3, 7, 14, 30 and 60 days (d), respectively. Following 60 days of ensiling, the silage was subjected to a 5-day period of aerobic exposure (AE). EPS changed the fermentation quality of silage, altered the composition of the bacterial community, and had an impact on the feature dissimilarity between sample groups. Meanwhile, EPS showed different regulatory effects on carbohydrate metabolism at different fermentation times. EPS treatment increased the lactic acid content and decreased the pH of silage. After 60 days of fermentation, the treatment also increased the relative abundance of Lactobacillus. Dextran and levan increased the relative abundance of Hafnia–Obesumbacterium and Sediminibacterium, respectively. Under the treatment of dextran, silage retained more WSC content and achieved higher aerobic stability. Upon comparing the bacterial correlation networks, it became evident that the fermentation time altered the composition of inter-bacterial correlations. In conclusion, EPS can effectively enhance the fermentation quality of oat silage, with dextran yielding the most pronounced positive effects.

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来源期刊
Microbial Biotechnology
Microbial Biotechnology Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
11.20
自引率
3.50%
发文量
162
审稿时长
1 months
期刊介绍: Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes
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