{"title":"无创漫反射光谱法测定新生儿胆红素的发展与验证。","authors":"Chitra R, Shanthi Prince, Ashok Chandrasekaran, Subash Sundar","doi":"10.1002/jbio.202400505","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Jaundice due to excess bilirubin buildup in neonates remains an alarming problem, especially during the very first week of postnatal life. The common method of measuring total serum bilirubin (TSB) levels involves invasive blood testing, which is time-consuming and uncomfortable for neonates. While noninvasive transcutaneous bilirubinometers (TcB) are available, that often lack accuracy due to interference from other biological chromophores. This study aims to improve bilirubin assessment by developing a noninvasive system that uses diffuse reflectance spectroscopy and a novel algorithm accounting for chromophores like melanin and hemoglobin. Focusing on the sternum as the measurement site, spectra are collected from 285 newborns. Using the proposed algorithm, the bilirubin concentration is calculated at 450, 460, and 470 nm. Compared to TSB levels, the proposed method demonstrated strong accuracy with an average bias of −0.10 mg/dL and narrower limits of agreement of 4.85 mg/dL at 470 nm.</p>\n </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 5","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and Validation of a Noninvasive Diffuse Reflectance Spectroscopic Method for Bilirubin Estimation in Neonates\",\"authors\":\"Chitra R, Shanthi Prince, Ashok Chandrasekaran, Subash Sundar\",\"doi\":\"10.1002/jbio.202400505\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Jaundice due to excess bilirubin buildup in neonates remains an alarming problem, especially during the very first week of postnatal life. The common method of measuring total serum bilirubin (TSB) levels involves invasive blood testing, which is time-consuming and uncomfortable for neonates. While noninvasive transcutaneous bilirubinometers (TcB) are available, that often lack accuracy due to interference from other biological chromophores. This study aims to improve bilirubin assessment by developing a noninvasive system that uses diffuse reflectance spectroscopy and a novel algorithm accounting for chromophores like melanin and hemoglobin. Focusing on the sternum as the measurement site, spectra are collected from 285 newborns. Using the proposed algorithm, the bilirubin concentration is calculated at 450, 460, and 470 nm. Compared to TSB levels, the proposed method demonstrated strong accuracy with an average bias of −0.10 mg/dL and narrower limits of agreement of 4.85 mg/dL at 470 nm.</p>\\n </div>\",\"PeriodicalId\":184,\"journal\":{\"name\":\"Journal of Biophotonics\",\"volume\":\"18 5\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-02-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biophotonics\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jbio.202400505\",\"RegionNum\":3,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biophotonics","FirstCategoryId":"101","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jbio.202400505","RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Development and Validation of a Noninvasive Diffuse Reflectance Spectroscopic Method for Bilirubin Estimation in Neonates
Jaundice due to excess bilirubin buildup in neonates remains an alarming problem, especially during the very first week of postnatal life. The common method of measuring total serum bilirubin (TSB) levels involves invasive blood testing, which is time-consuming and uncomfortable for neonates. While noninvasive transcutaneous bilirubinometers (TcB) are available, that often lack accuracy due to interference from other biological chromophores. This study aims to improve bilirubin assessment by developing a noninvasive system that uses diffuse reflectance spectroscopy and a novel algorithm accounting for chromophores like melanin and hemoglobin. Focusing on the sternum as the measurement site, spectra are collected from 285 newborns. Using the proposed algorithm, the bilirubin concentration is calculated at 450, 460, and 470 nm. Compared to TSB levels, the proposed method demonstrated strong accuracy with an average bias of −0.10 mg/dL and narrower limits of agreement of 4.85 mg/dL at 470 nm.
期刊介绍:
The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.