Mengge Li, Qimeng Heng, Xinyang Yan, Mengfan Guo, Zhaoming Liu, Zheng Chen, Tao Gao, Xuelian He, Zheyuan Zhang, Yinglong Chen, Jean W H Yong, Rongkai Wang, Junfeng Fan, Yi Zhang
{"title":"Expression complementation between fundamental biological pathways in Populus hybrid contributes to heterosis in cadmium (Cd) accumulation and tolerance.","authors":"Mengge Li, Qimeng Heng, Xinyang Yan, Mengfan Guo, Zhaoming Liu, Zheng Chen, Tao Gao, Xuelian He, Zheyuan Zhang, Yinglong Chen, Jean W H Yong, Rongkai Wang, Junfeng Fan, Yi Zhang","doi":"10.1093/treephys/tpaf025","DOIUrl":null,"url":null,"abstract":"<p><p>To reveal the pattern of heterosis in cadmium (Cd) bio-accumulation of poplar and whether the heterosis can promote the phytoremediation efficiency of Cd-polluted soil, the poplar hybrid variety QB-5 (I-101 × 84K) and its female parent I-101 (Populus alba) and male parent 84K (P. alba × P. glandulosa) were employed in a hydroponic experiment and a field trial. BPHs of leaf biomass, leaf area, free proline, CAT, SA and Cd bio-accumulation reached 100.30%, 97.23%, 57.96% 176.41%, 102.94% and 164.17%, respectively, under Cd exposure. A more in-depth analysis unveiled that most traits related to Cd bio-concentration, including root parameters, Cd translocation factor (TF), and Cd bioconcentration factor (BCF) in leaves, were dominant in 84K. In contrast, traits related to stress tolerance were dominant in I-101. Weighted gene co-expression network analysis (WGCNA) revealed that hub genes responsible for Cd translocation and bioconcentration were dominantly expressed in 84K, resulting in superior leaf Cd concentration in males compared to females. Conversely, most genes responsible for stress tolerance were highly expressed in I-101. The hybrid exhibited a high-parent complementation pattern for critical traits and relevant hub genes, contributing to better-parent heterosis for these traits. Overexpression of PagP5CS1, a gene showing above-high-parent expression in hybrid, increased Cd tolerance and Cd bio-accumulation in poplar, providing molecular evidence for the dominance hypothesis of heterosis. The efficiency of phytoremediation for Cd-contaminated soil can be largely promoted by exploring and utilizing heterosis in Cd tolerance and Cd bio-accumulation.</p>","PeriodicalId":23286,"journal":{"name":"Tree physiology","volume":" ","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tree physiology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/treephys/tpaf025","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FORESTRY","Score":null,"Total":0}
Expression complementation between fundamental biological pathways in Populus hybrid contributes to heterosis in cadmium (Cd) accumulation and tolerance.
To reveal the pattern of heterosis in cadmium (Cd) bio-accumulation of poplar and whether the heterosis can promote the phytoremediation efficiency of Cd-polluted soil, the poplar hybrid variety QB-5 (I-101 × 84K) and its female parent I-101 (Populus alba) and male parent 84K (P. alba × P. glandulosa) were employed in a hydroponic experiment and a field trial. BPHs of leaf biomass, leaf area, free proline, CAT, SA and Cd bio-accumulation reached 100.30%, 97.23%, 57.96% 176.41%, 102.94% and 164.17%, respectively, under Cd exposure. A more in-depth analysis unveiled that most traits related to Cd bio-concentration, including root parameters, Cd translocation factor (TF), and Cd bioconcentration factor (BCF) in leaves, were dominant in 84K. In contrast, traits related to stress tolerance were dominant in I-101. Weighted gene co-expression network analysis (WGCNA) revealed that hub genes responsible for Cd translocation and bioconcentration were dominantly expressed in 84K, resulting in superior leaf Cd concentration in males compared to females. Conversely, most genes responsible for stress tolerance were highly expressed in I-101. The hybrid exhibited a high-parent complementation pattern for critical traits and relevant hub genes, contributing to better-parent heterosis for these traits. Overexpression of PagP5CS1, a gene showing above-high-parent expression in hybrid, increased Cd tolerance and Cd bio-accumulation in poplar, providing molecular evidence for the dominance hypothesis of heterosis. The efficiency of phytoremediation for Cd-contaminated soil can be largely promoted by exploring and utilizing heterosis in Cd tolerance and Cd bio-accumulation.
期刊介绍：
Tree Physiology promotes research in a framework of hierarchically organized systems, measuring insight by the ability to link adjacent layers: thus, investigated tree physiology phenomenon should seek mechanistic explanation in finer-scale phenomena as well as seek significance in larger scale phenomena (Passioura 1979). A phenomenon not linked downscale is merely descriptive; an observation not linked upscale, might be trivial. Physiologists often refer qualitatively to processes at finer or coarser scale than the scale of their observation, and studies formally directed at three, or even two adjacent scales are rare. To emphasize the importance of relating mechanisms to coarser scale function, Tree Physiology will highlight papers doing so particularly well as feature papers.
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