卵母细胞sumo酰化的破坏影响小鼠卵泡发生过程中的关键调控过程。

IF 3.1 2区 生物学 Q2 REPRODUCTIVE BIOLOGY
Zian Liao, Tessa E Steenwinkel, Bruno Moscoso, Ernesto Salas, Bethany K Patton, Amanda Rodriguez, Anna Malovannaya, Stephanie A Pangas
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引用次数: 0

摘要

小泛素样修饰物(SUMO)与靶蛋白的结合,被称为SUMO化,在调节蛋白质稳态、活性、与其他蛋白质的相互作用和亚细胞定位中起着至关重要的作用。原始卵泡期E2相扑偶联酶Ube2i的条件缺失导致非生长卵母细胞中SUMOylation的缺失,导致雌性不育,原因是卵母细胞发育发生了复杂的变化,包括卵泡发生改变、减数分裂过程缺陷和卵巢储备的过早丧失。在这项研究中,蛋白质组学被用于比较在卵泡发生的第一波中,对照组和Ube2i条件敲除卵巢,以确定可能驱动过早卵泡丢失表型的关键差异。数据可通过ProteomeXchange获得,标识符为PXD055913。无标记质谱分析结果显示,238个蛋白显著改变超过2倍(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Disruption of oocyte SUMOylation impacts critical regulatory processes during folliculogenesis in mice†.

The conjugation of small ubiquitin-like modifiers (SUMO) to target proteins, known as SUMOylation, plays a crucial role in regulating protein homeostasis, activity, interaction with other proteins, and subcellular localization. Loss of SUMOylation in nongrowing oocytes by conditional deletion of the E2 SUMO conjugating enzyme, Ube2i, at the primordial follicle stage leads to female sterility due to complex changes in oocyte development, including altered folliculogenesis, defective meiotic progression, and premature loss of the ovarian reserve. In this study, proteomics was used to compare control and Ube2i conditional knockout ovaries during the first wave of folliculogenesis to identify key differences that may drive the premature follicle loss phenotype. Label-free mass spectrometry results showed that 238 proteins were significantly altered more than 2-fold (p < 0.05). Proteins upregulated in the Ube2i conditional knockout ovaries included those involved in mRNA splicing and WNT signaling, while those downregulated were related to metabolism, mitochondria, and the maternal effect proteins NLRP2 and NLRP9B. The majority of differentially expressed proteins showed no change by transcriptome analysis, indicating protein level regulation and revealing potential SUMOylation targets with necessary roles in oocyte and follicle development.

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来源期刊
Biology of Reproduction
Biology of Reproduction 生物-生殖生物学
CiteScore
6.30
自引率
5.60%
发文量
214
审稿时长
1 months
期刊介绍: Biology of Reproduction (BOR) is the official journal of the Society for the Study of Reproduction and publishes original research on a broad range of topics in the field of reproductive biology, as well as reviews on topics of current importance or controversy. BOR is consistently one of the most highly cited journals publishing original research in the field of reproductive biology.
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