常压冷等离子体处理对海藻酸豌豆分离蛋白电荷、结构、热性能及凝聚行为的影响

IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Prashant Kumar , P.S.N.S.R. Srikar , Dhananjay Dahatonde , Reetesh Kumar Gangwar , Trivikram Nallamilli
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引用次数: 0

摘要

本研究探讨了豌豆分离蛋白(PPI)和海藻酸盐(AG)在复杂凝聚中的特性和相互作用,重点研究了蛋白-多糖混合物在凝聚前冷等离子体(CP)处理的影响以及不同pH水平的影响。CP处理时间分别为5、10、15和20分钟,并通过发射光谱(OES)和电流-电压测量分析等离子体特性。非热多针电晕放电的发射光谱显示,气体温度为330±5 K,电子密度为(2.6±0.1)× 1021 m−3,有明显的OH (a - x)和N2 (CB)发射。CP处理后,用浊度、Zeta电位、粒径、傅立叶红外光谱(FTIR)、热分析(TGA和DSC)和等温滴定量热法(ITC)对凝聚物进行表征。CP处理导致颗粒大小降低约33.4%,透明度提高,如浊度降低所示。Zeta电位分析表明,在pH为4时,PPI-AG(2:1)在20 min后负电荷增加41.7%,增强了稳定性和凝聚效率。FTIR分析显示,在pH为4.5时,PPI-AG(1:1)复合物中β-片减少了25.56%,α-螺旋增加了52.34%,β-匝数增加了41.66%。热分析表明,在pH值为4.5时,PPI-AG(1:1)复合物的稳定性降低了9.4%,同时保水能力增强。ITC显示,在较低ph下处理的样品中,放热相互作用更强。总体而言,CP处理改善了PPI-AG配合物的物理化学性质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of atmospheric pressure cold plasma treatment on charge, structure, thermal properties and coacervation behavior of Pea protein isolate with Alginate
This study explores the characteristics and interactions of pea protein isolate (PPI) and alginate (AG) in complex coacervation, focusing on the effects of cold plasma (CP) treatment applied to the protein-polysaccharide mixture before coacervation and the influence of varying pH levels. CP treatment was applied at different durations (5, 10, 15, and 20 min) and with plasma characteristics analyzed through optical emission spectroscopy (OES) and current-voltage measurements. Emission spectroscopy of the nonthermal multi-needle corona discharge revealed prominent OH (A-X) and N2 (CB) emissions with a gas temperature of 330 ± 5 K and electron density of (2.6 ± 0.1) × 1021 m−3. Following CP treatment, the coacervates were characterized using Turbidity, Zeta potential, Particle size, Fourier Transform Infrared Spectroscopy (FTIR), Thermal Analysis (TGA and DSC), and Isothermal Titration Calorimetry (ITC). CP treatment resulted in a ≈ 33.4 % reduction in particle size and improved transparency, as indicated by reduced turbidity. Zeta potential analysis showed a 41.7 % increase in the negative charge for PPI-AG (2:1) at pH 4 after 20 min, enhancing the stability and coacervation efficiency. FTIR analysis indicated a 25.56 % reduction in β-sheets, with a 52.34 % increase in α-helices and a 41.66 % increase in β-turns in the PPI-AG (1:1) complex at pH 4.5 after 10 min of CP treatment. Thermal analysis showed a 9.4 % decrease in stability for the PPI-AG (1:1) complex at pH 4.5, along with enhanced water-holding capacity. ITC revealed stronger exothermic interactions in treated samples at lower pH. Overall, CP treatment improved the physicochemical properties of PPI-AG complexes.
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来源期刊
CiteScore
12.00
自引率
6.10%
发文量
259
审稿时长
25 days
期刊介绍: Innovative Food Science and Emerging Technologies (IFSET) aims to provide the highest quality original contributions and few, mainly upon invitation, reviews on and highly innovative developments in food science and emerging food process technologies. The significance of the results either for the science community or for industrial R&D groups must be specified. Papers submitted must be of highest scientific quality and only those advancing current scientific knowledge and understanding or with technical relevance will be considered.
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