麦卡蒂脱盐coccoides mccartyi菌株CWV2脱氯机理研究：多组学视角

IF 12.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-02-20 DOI:10.1016/j.watres.2025.123347

Chih-Ming Kao , Ku-Fan Chen , Po-Sheng Kuo , Chih-Ching Chien , Che-Wei Lu , Ssu-Ching Chen

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引用次数: 0

摘要

摘要本研究从台湾分离的麦卡蒂dehalococoides mccartyi （Dhc）菌株CWV2，对其对多种氯乙烯（包括PCE、TCE、dce和VC）的脱氯作用进行了深入研究。通过多组学分析，包括基因组学、转录组学、翻译组学和蛋白质组学分析，我们揭示了菌株CWV2脱氯TCE的机制。基因组分析确定了还原脱卤酶（RDase）的关键基因pceA和vcrA，从而加深了我们对Dhc多种脱氯途径的理解。核糖体分析为vcrA的翻译调控提供了详细的见解，揭示了蛋白质合成的复杂遗传控制。补充BN-PAGE和蛋白质组学分析确定了关键的rda酶VcrA，进一步了解了CWV2中有机卤化物呼吸（OHR）复合物的活性及其代谢途径。多组学分析提供了对TCE脱氯和有机卤化物呼吸背后机制的全面理解，为推进氯乙烯污染环境的生物修复策略提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Mechanistic insights into chloroethene dechlorination by Dehalococcoides mccartyi strain CWV2: A multi-omics perspective

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Mechanistic insights into chloroethene dechlorination by Dehalococcoides mccartyi strain CWV2: A multi-omics perspective

This study provides an in-depth investigation of the novel Dehalococcoides mccartyi (Dhc) strain CWV2, isolated from Taiwan, for its effectiveness in dechlorinating various chloroethenes, including PCE, TCE, DCEs, and VC, to ethene. Through multi-omics analyses, including genomic, transcriptomic, translatomic and proteomic profiling, we uncovered the mechanisms behind TCE dechlorination by strain CWV2. The genomic analysis identified key reductive dehalogenase (RDase) genes, pceA and vcrA, which enhance our understanding of the versatile dechlorination pathways in Dhc. Ribosome profiling provided detailed insights into the translational regulation of vcrA, revealing sophisticated genetic control over protein synthesis. Complementary BN-PAGE and proteomic analyses identified key RDase VcrA, offering further insights into the activity of the organohalide respiration (OHR) complex within CWV2 and its metabolic pathways. Multi-omics analyses provide a comprehensive understanding of the mechanisms behind TCE dechlorination and organohalide respiration, offering valuable insights to advance bioremediation strategies for chloroethene-contaminated environments.

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.