用深共晶溶剂合成聚吡咯-氧化石墨烯及其荧光光谱法测定甲硝唑原料药的表征

IF 2 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Hani K. Ismail, Aryan F. Qader, Rebaz A. Omer, Hasan F. Alesary, Haider M. Umran, Aseel A. Kareem
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引用次数: 0

摘要

在氯化胆碱和丙酸的摩尔比为1:2的深共晶溶剂中,采用化学聚合法制备了聚吡咯-氧化石墨烯纳米复合材料。本研究的目的是研究聚吡咯/氧化石墨烯纳米复合材料在利用荧光光谱技术检测药物配方中甲硝唑的可行性和有效性。采用透射电子显微镜(TEM)、扫描电子显微镜(SEM)、傅里叶变换红外光谱(FTIR)、热重分析(TGA)和x射线衍射(XRD)等方法对合成的聚吡咯/氧化石墨烯纳米复合材料进行表征。考察了pH缓冲液、潜在干扰、反应时间、甲硝唑浓度、干扰等最佳参数,并在实际样品中进行了试验。在建立的理想条件下,标定曲线在0.3 ~ 20µg/mL范围内呈线性关系,相关系数(R2)为0.98712。检测限(LOD)为0.09µg/mL,定量限(LOQ)为0.29µg/mL。测定了3种甲硝唑标准溶液(0.3、10、20µg/mL) 5个重复的相对标准偏差(RSD)读数,RSD百分比为1.29% ~ 2.34%,误差百分比为- 3.33% ~ 1.20%,证明了该方法的精密度和准确性。本研究为药物样品中甲硝唑的检测提供了一种可行而有效的方法。此外,这些数据表明,聚吡咯/氧化石墨烯可能构成一种新型的荧光猝灭试剂,可用于医学应用。该方法对常规分析和质量控制分析是有效的,因为它相对不受干扰。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Synthesis of Polypyrrole-Graphene Oxide (PPy/GO) from Deep Eutectic Solvent and Its Characterization for Determination of Metronidazole Pharmaceutical Substance Using Spectrofluorometric Technique

Synthesis of Polypyrrole-Graphene Oxide (PPy/GO) from Deep Eutectic Solvent and Its Characterization for Determination of Metronidazole Pharmaceutical Substance Using Spectrofluorometric Technique

Synthesis of Polypyrrole-Graphene Oxide (PPy/GO) from Deep Eutectic Solvent and Its Characterization for Determination of Metronidazole Pharmaceutical Substance Using Spectrofluorometric Technique

Synthesis of Polypyrrole-Graphene Oxide (PPy/GO) from Deep Eutectic Solvent and Its Characterization for Determination of Metronidazole Pharmaceutical Substance Using Spectrofluorometric Technique

A polypyrrole-graphene oxide (PPy/GO) nanocomposite was made chemically using a polymerization method in deep eutectic solvents made up of choline chloride and propionic acid in a 1:2 molar ratio. The purpose of this study is to examine the feasibility and efficacy of PPy/GO nanocomposite in the detection of metronidazole in pharmaceutical formulations utilizing spectrofluorometric techniques. Characterization of the synthesized PPy/GO nanocomposite was conducted using several methods such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), and X-ray diffraction (XRD). The optimal parameters such as pH buffer solution, potential interferences, reaction duration, concentrations of metronidazole, and interference, and test in real sample were examined. Under the established ideal conditions, the curve of calibration exhibited a linear range of 0.3–20 µg/mL, with a correlation coefficient (R2) of 0.98712. The limit of detection (LOD) was 0.09 µg/mL, while the limit of quantification (LOQ) was 0.29 µg/mL. The relative standard deviation (RSD) readings for five replicates of three metronidazole standard solutions (0.3, 10, or 20 µg/mL) were assessed, yielding RSD percentages ranging from 1.29% to 2.34% and error percentages from −3.33% to 1.20%, thereby demonstrating the method's precision and accuracy. This study provides a viable and useful way to detect metronidazole in pharmaceutical samples. Moreover, the data indicate that PPy/GO may constitute a novel category of fluorescence quenching reagent for medicinal applications. This approach is effective for regular analysis and quality control assays because of its relative freedom from interferences.

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来源期刊
ChemistrySelect
ChemistrySelect Chemistry-General Chemistry
CiteScore
3.30
自引率
4.80%
发文量
1809
审稿时长
1.6 months
期刊介绍: ChemistrySelect is the latest journal from ChemPubSoc Europe and Wiley-VCH. It offers researchers a quality society-owned journal in which to publish their work in all areas of chemistry. Manuscripts are evaluated by active researchers to ensure they add meaningfully to the scientific literature, and those accepted are processed quickly to ensure rapid online publication.
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