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IF 3.2 4区医学 Q2 NEUROSCIENCES

Autonomic Neuroscience-Basic & Clinical Pub Date : 2025-02-15 DOI:10.1016/j.autneu.2025.103252

Amanda Barbosa Coelho da Silva , Stefanne Madalena Marques , James O. Fajemiroye , Eduardo Colombari , Carlos Henrique Xavier , Marcos Luiz Ferreira-Neto , Lara Marques Naves , Gustavo Rodrigues Pedrino

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引用次数: 0

摘要

背景研究报道了使用高渗盐水（HSS）治疗低血压性出血（HH）。尽管中枢机制在 HSS 诱导的 HH 期间心血管恢复中的作用已经确立，但角膜下器官（SFO）在这些反应中的参与仍有待阐明。本研究评估了 SFO 和细胞核中的肾上腺素能神经传递在出血大鼠输注 HSS 的心血管反应中的作用。方法记录 Wistar 大鼠的平均动脉压（MAP）、心率（HR）和主动脉血管阻力（AVR）。通过抽血进行 HH，直到 MAP 达到 60 mmHg。抽血开始 10 分钟后，在 SFO 中注射生理盐水（NaCl；0.15 M；对照组；n = 7）、麝香草酚（4 mM；GABA 能激动剂；麝香草酚组；n = 7）或普萘洛尔（10 mM；非选择性 β 肾上腺素能阻断剂；普萘洛尔组；n = 7），然后输注 HSS（NaCl；3 M；1.结果在 HH 期间，所有组均观察到低血压、心动过缓和主动脉血管收缩。钠超载可恢复血压和心率，同时维持对照组的主动脉血管收缩。在 SFO 中激活 GABA A 受体或阻断 β 肾上腺素能受体可防止 HSS 诱导的 MAP 和 HR 恢复。结论结果表明，SFO 神经元和 β 肾上腺素能神经递质的完整性对出血大鼠钠超载引起的心血管恢复至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Hypertonic saline solution evokes cardiovascular recovery in hemorrhagic rats dependent on GABA A and β-adrenergic transmission in the subfornical organ

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Hypertonic saline solution evokes cardiovascular recovery in hemorrhagic rats dependent on GABA A and β-adrenergic transmission in the subfornical organ

Background

Studies have reported the use of a hypertonic saline solution (HSS) for the treatment of hypotensive hemorrhage (HH). Despite the established role of central mechanisms in the cardiovascular recovery induced by HSS during HH, the involvement of the Subfornical Organ (SFO) in these responses remains to be elucidated. The present study evaluated the role of SFO and adrenergic neurotransmission in the nucleus in the cardiovascular responses to HSS infusion in hemorrhagic rats.

Methods

Mean arterial pressure (MAP), heart rate (HR), and aortic vascular resistance (AVR) were recorded in Wistar rats. HH was performed through blood withdrawal until a MAP of 60 mmHg was attained. Nanoinjections of saline (NaCl; 0.15 M; control group; n = 7), muscimol (4 mM; GABAergic agonist; muscimol group; n = 7), or propranolol (10 mM; non-selective β-adrenergic blocker; propranolol group; n = 7) in SFO were performed 10 min after the onset of blood withdrawal, followed by HSS infusion (NaCl; 3 M; 1.8 ml∙kg⁻¹) 20 min after the beginning of HH.

Results

Hypotension, bradycardia, and aortic vasoconstriction were observed in all groups During HH. Sodium overload reestablished MAP and HR while maintaining aortic vasoconstriction in the control group. Activation of GABA A receptors or β-adrenergic receptor blockade in the SFO prevents HSS-induced recovery of MAP and HR. In addition, maintenance of aortic vasoconstriction induced by HSS infusion was abolished by SFO inhibition.

Conclusions

The results suggest that the integrity of SFO neurons and β-adrenergic neurotransmission are essential for cardiovascular recovery promoted by sodium overload in hemorrhagic rats.

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来源期刊

Autonomic Neuroscience-Basic & Clinical 医学-神经科学

CiteScore

5.80

自引率

7.40%

发文量

审稿时长

66 days

期刊介绍： This is an international journal with broad coverage of all aspects of the autonomic nervous system in man and animals. The main areas of interest include the innervation of blood vessels and viscera, autonomic ganglia, efferent and afferent autonomic pathways, and autonomic nuclei and pathways in the central nervous system. The Editors will consider papers that deal with any aspect of the autonomic nervous system, including structure, physiology, pharmacology, biochemistry, development, evolution, ageing, behavioural aspects, integrative role and influence on emotional and physical states of the body. Interdisciplinary studies will be encouraged. Studies dealing with human pathology will be also welcome.