纳米磁铁矿纳米酶在微生物纤维素水凝胶上固定化活化过硫酸盐的抑菌效果

IF 5.5 3区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of the Taiwan Institute of Chemical Engineers Pub Date : 2025-02-21 DOI:10.1016/j.jtice.2025.106039

Shabnam Ahmadi , Mohammad Hadi Dehghani , Abbas Rezaee

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引用次数: 0

摘要

本研究以微生物纤维素水凝胶(MCH)/纳米磁铁矿（Fe3O4）纳米颗粒（NPs）制备的纳米复合材料为纳米酶，研究了活化过硫酸盐（PS）的抗菌活性。方法考察纳米复合材料浓度、过硫酸盐浓度、pH值等主要反应参数。利用动态光散射（DLS）、傅里叶变换红外光谱（FTIR）、x射线粉末衍射（XRD）、场发射扫描电镜（FE-SEM）和振动样品磁强计（VSM）等多种分析方法对纳米复合材料和纳米酶进行了分析。结果表明，纳米复合材料在最佳条件下（温度：25℃，纳米酶浓度：2 mg/mL，溶液pH: 4.0）具有抗菌活性。此外，在0.25 g/l PS条件下，纳米复合材料的抗菌活性有所提高，对3,3 ',5,5 ' -四甲基联苯胺（TMB）的Michaelis常数（Km）为0.32 mM，最大反应速度（Vmax）为0.6 10−8 mM sec−1。抗菌活性增加的原因是由于产生了更高的活性氧（ROS），提高了大肠杆菌的消除。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Antimicrobial effect based on activated persulfate using nano-magnetite nanozyme immobilized on the microbial cellulose hydrogel

查看原文本刊更多论文

Antimicrobial effect based on activated persulfate using nano-magnetite nanozyme immobilized on the microbial cellulose hydrogel

Background

The present study is to investigate the antimicrobial activity of the activated persulfate (PS) using the nanocomposite fabricated by microbial cellulose hydrogel (MCH)/nanomagnetite (Fe₃O₄) nanoparticles (NPs) as a nanozyme.

Methods

The main reaction parameters including nanocomposite concentration, persulfate concentration and pH values were investigated. Various analysis, such as dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), field emission-scanning electron microscopy (FE-SEM), and vibrating-sample magnetometry (VSM), were utilized to analyze the nanocomposite and the nanozyme.

Significant findings

The obtained results show that the nanocomposite has antimicrobial activity under optimum conditions (temperature: 25 °C, nanozyme concentration: 2 mg/mL, and solution pH: 4.0). Moreover, increasing of the antimicrobial activity of the nanocomposite is observed using 0.25 g/l PS. The nanozyme exhibits the Michaelis constant (K_m) of 0.32 mM and the maximum reaction velocity (V_max) of 0.6 10⁻⁸ mM sec⁻¹ for 3,3′,5,5′-tetramethylbenzidine (TMB). The reason for the increasing of the antimicrobial activity is due to the generation of higher active oxygen species (ROS), which improve the elimination of the E. coli.

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来源期刊

Journal of the Taiwan Institute of Chemical Engineers 工程技术-工程：化工

CiteScore

9.10

自引率

14.00%

发文量

362

审稿时长

35 days

期刊介绍： Journal of the Taiwan Institute of Chemical Engineers (formerly known as Journal of the Chinese Institute of Chemical Engineers) publishes original works, from fundamental principles to practical applications, in the broad field of chemical engineering with special focus on three aspects: Chemical and Biomolecular Science and Technology, Energy and Environmental Science and Technology, and Materials Science and Technology. Authors should choose for their manuscript an appropriate aspect section and a few related classifications when submitting to the journal online.