微氧和酸性条件下嗜氧和嗜酸铁氧化菌高效硫化铁氧化固定化砷的新见解

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-02-20 DOI:10.1016/j.jhazmat.2025.137695

Mengyao Li, Zhe Wang, Zekai Feng, Jianan Lu, Daijie Chen, Chiyu Chen, Huan He, Qi Zhang, Xiaoting Chen, Jean Louis Morel, Alan J M Baker, Yuanqing Chao, Yetao Tang, Feng Jiang, Rongliang Qiu, Shizhong Wang

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引用次数: 0

摘要

在pH/O₂限制下，微生物介导的FeS通过化化岩自养Fe（Ⅱ）氧化剂氧化为Fe（Ⅲ）矿物，涉及As固定化。然而，由于铁（Ⅱ）氧化能力的严重降低，该过程在微氧和酸性条件下的双重应力下受到限制。因此，在微氧和酸性条件下，Fe（Ⅱ）氧化细菌中的Fe（Ⅱ）氧化、碳代谢和As固定化之间的相互作用尚不清楚。本研究首次成功富集了嗜微氧和亲酸的铁（II）氧化细菌（MAFeOB）。这些细菌能够在微氧含量（低于3.2 mg/L）和酸性pH（4.5-6.2）下将FeS氧化为Fe（III）矿物，并固定化高达27,835 mg/kg的As（Ⅴ）。通过全面的宏基因组分析，推测MAFeOB含有一套可能参与关键过程的基因，包括碳固定、铁（II）氧化和砷解毒。值得注意的是，从Cyc2_repCluster2到细胞色素cbb3型氧化酶的潜在电子转移途径促进了Fe（II）的氧化。此外，As（Ⅲ）外排泵（arsA, arsB, acr3）和As（Ⅲ）氧化酶（aioA）基因表明MAFeOB具有固定As的潜力。我们的发现强调了MAFeOB在克服与Fe（III）矿物形成相关的限制方面的关键作用，从而增强了微氧和酸性水下砷的固定化。

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New insights into efficient iron sulfide oxidation for arsenic immobilization by microaerophilic and acidophilic Fe(II)-oxidizing bacteria under micro-oxygen and acidic conditions

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New insights into efficient iron sulfide oxidation for arsenic immobilization by microaerophilic and acidophilic Fe(II)-oxidizing bacteria under micro-oxygen and acidic conditions

Microbial-mediated FeS oxidation to Fe(Ⅲ) minerals via chemolithoautotrophic Fe(Ⅱ) oxidizers under pH/O₂ limitations engages As immobilization. However, this process is constrained under the dual stress of micro-oxygen and acidic conditions due to the critically diminished Fe(Ⅱ) oxidation capacity. Therefore, the interplay between Fe(Ⅱ) oxidation, carbon metabolism, and As immobilization in Fe(Ⅱ)-oxidizing bacteria under micro-oxygen and acidic conditions remains unclear. This study presents the first successful enrichment of microaerophilic and acidophilic Fe(II)-oxidizing bacteria (MAFeOB). These bacteria are capable of oxidizing FeS to Fe(III) minerals and immobilizing up to 27,835 mg/kg of As(Ⅴ) under micro-oxygen content (below 3.2 mg/L) and acidic pH (4.5-6.2). Through comprehensive metagenomic analysis, it was speculated that MAFeOB harbor a suite of genes potentially participating in critical processes, including carbon fixation, Fe(II) oxidation, and arsenic detoxification. Notably, a potential electron transfer pathway from Cyc2_repCluster2 to Cytochrome cbb3-type oxidases facilitates Fe(II) oxidation. Furthermore, As(Ⅲ) efflux pump (arsA, arsB, acr3) and As(Ⅲ) oxidase (aioA) genes indicate MAFeOB's potential for As immobilization. Our findings underscore the pivotal role of MAFeOB in overcoming limitations associated with Fe(III) mineral formation, thereby enhancing arsenic immobilization under micro-oxygen and acidic water.

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.