人Ewing肉瘤细胞系胃泌素前释放肽的亚细胞定位。

IF 1.1 4区 医学 Q3 PATHOLOGY
Medical Molecular Morphology Pub Date : 2025-09-01 Epub Date: 2025-02-18 DOI:10.1007/s00795-025-00426-2
Koji Muramatsu, Kouji Maruyama, Yosuke Honda, Hirohisa Katagiri, Yu Takahashi, Naoki Takahashi, Katsumi Aoyagi, Kenichi Mori, Mitsuru Takahashi, Toru Kameya, Takashi Sugino, Ken Yamaguchi
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引用次数: 0

摘要

原胃泌素释放肽(Progastrin-releasing peptide, ProGRP)是一种神经肽,是小细胞肺癌(SCLC)和其他神经内分泌肿瘤(如甲状腺髓样癌)的稳定肿瘤标志物。最近有报道称它在尤文氏肉瘤(ES)中高度表达,并作为这类肿瘤的有用标记物。然而,ProGRP产生的机制尚不清楚。本研究旨在阐明其在人胚胎干细胞系中的亚细胞定位。将3个ES细胞系(A-673、SK-N-MC和SK-NEP-1)和1个SCLC细胞系(DMS 53)移植到胸腺小鼠体内。术后3 ~ 4周切除肿瘤,采用免疫组化和免疫电镜检测ProGRP的表达和定位。免疫组化结果显示,三种ES细胞系细胞质中ProGRP的表达呈点状,而SCLC细胞则呈弥漫性颗粒状。免疫电镜显示,ProGRP定位于SCLC细胞的神经分泌颗粒(nsg)和胚胎干细胞的自溶酶体样结构中,而在胚胎干细胞系中未观察到nsg。胚胎干细胞分泌高水平的ProGRP,类似于SCLC细胞。然而,在ES细胞中,与SCLC中的nsg不同,ProGRP定位于自溶酶体样结构,这表明ES中的ProGRP可以不遵循神经肽的典型途径运输和分泌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Subcellular localization of pro-gastrin releasing peptide in human Ewing sarcoma cell lines.

Progastrin-releasing peptide (ProGRP), a neuropeptide, is a stable tumor marker for small cell lung carcinoma (SCLC) and other neuroendocrine tumors, such as medullary thyroid carcinoma. It has recently been reported as highly expressed in Ewing sarcoma (ES) and as a useful marker for this type of tumor. However, the mechanisms underlying ProGRP production remain unclear. This study aimed to elucidate its subcellular localization in human ES cell lines. Three ES cell lines (A-673, SK-N-MC, and SK-NEP-1) and one SCLC line (DMS 53) were transplanted into athymic mice. After 3-4 weeks, the tumors were excised, and the expression and localization of ProGRP were examined using immunohistochemistry and immunoelectron microscopy. Immunohistochemistry revealed that all three ES cell lines expressed ProGRP in a dot-like pattern in their cytoplasm, whereas SCLC cells showed a diffuse granular pattern. Immunoelectron microscopy revealed that ProGRP localized in neurosecretory granules (NSGs) in SCLC cells and in autolysosome-like structures in ES cells, with no NSGs observed in the ES cell lines. ES cells secrete high levels of ProGRP, similar to SCLC cells. However, in ES cells, ProGRP is localized in autolysosome-like structures, unlike the NSGs in SCLC, suggesting that ProGRP in ES can be transported and secreted without following the typical pathway for neuropeptides.

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来源期刊
Medical Molecular Morphology
Medical Molecular Morphology 医学-病理学
CiteScore
2.90
自引率
5.60%
发文量
30
审稿时长
>12 weeks
期刊介绍: Medical Molecular Morphology is an international forum for researchers in both basic and clinical medicine to present and discuss new research on the structural mechanisms and the processes of health and disease at the molecular level. The structures of molecules, organelles, cells, tissues, and organs determine their normal function. Disease is thus best understood in terms of structural changes in these different levels of biological organization, especially in molecules and molecular interactions as well as the cellular localization of chemical components. Medical Molecular Morphology welcomes articles on basic or clinical research in the fields of cell biology, molecular biology, and medical, veterinary, and dental sciences using techniques for structural research such as electron microscopy, confocal laser scanning microscopy, enzyme histochemistry, immunohistochemistry, radioautography, X-ray microanalysis, and in situ hybridization. Manuscripts submitted for publication must contain a statement to the effect that all human studies have been reviewed by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in an appropriate version of the 1964 Declaration of Helsinki. It should also be stated clearly in the text that all persons gave their informed consent prior to their inclusion in the study. Details that might disclose the identity of the subjects under study should be omitted.
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