滑膜液钙保护蛋白诊断假体周围关节感染的分析与临床考虑。

IF 6.6 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Mohammed F Alkadhem, Paul C Jutte, Marjan Wouthuyzen-Bakker, Anneke C Muller Kobold
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引用次数: 0

摘要

钙保护蛋白是一种主要存在于骨髓细胞(如中性粒细胞和单核细胞)细胞质中的蛋白质。钙保护蛋白在先天免疫中有多种功能,如削弱细菌,招募和激活免疫细胞,帮助产生促炎细胞因子和活性氧。由于其存在于炎症部位,它已被研究作为各种疾病的生物标志物,特别是炎症性肠病(IBD),类风湿性关节炎(RA),并在几种感染性疾病的诊断中获得了兴趣,特别是诊断假体周围关节感染(PJI)。滑液钙保护蛋白已被证明是一种敏感和特异性的生物标志物,可用于确认和排除PJI。滑液钙保护蛋白可用酶联免疫吸附试验(ELISA)、免疫比浊法和侧流法测定。它是一种稳定的生物标志物,无论血液或脂质污染、储存时间、冻融循环和酶促降粘预处理,其水平都不会显著降低或增加。本文就钙保护蛋白的生物学和生理学、PJI的病理生理学以及其在PJI诊断中的临床和分析考虑作一综述。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analytical and clinical considerations of synovial fluid calprotectin in diagnosing periprosthetic joint infections.

Calprotectin is a protein predominantly found in the cytosol of myeloid cells, such as neutrophils and monocytes. Calprotectin has several functions in innate immunity, such as attenuating bacteria, recruiting and activating immune cells, and aiding in the production of pro-inflammatory cytokines and reactive oxygen species. Due to its presence in inflammatory sites, it has been investigated as a biomarker for various medical conditions, especially Inflammatory bowel diseases (IBD), rheumatoid arthritis (RA), and has gained interest in the diagnosis of several infectious diseases, in particular for diagnosing periprosthetic joint infections (PJI). Synovial fluid calprotectin has demonstrated to be a sensitive and specific biomarker for both confirming as well as excluding PJI. Synovial fluid calprotectin can be measured using enzyme-linked immunosorbent assay (ELISA), immunoturbidimetry, and lateral flow methods. It is a generally stable biomarker, showing no significant decrease or increase in its levels despite blood or lipid contamination, storage duration, freeze-thaw cycles, and enzymatic pretreatments for viscosity reduction. This review discusses the biology and physiology of calprotectin, pathophysiology of PJI, and the clinical and analytical considerations surrounding its use in diagnosing PJI.

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来源期刊
CiteScore
20.00
自引率
0.00%
发文量
25
审稿时长
>12 weeks
期刊介绍: Critical Reviews in Clinical Laboratory Sciences publishes comprehensive and high quality review articles in all areas of clinical laboratory science, including clinical biochemistry, hematology, microbiology, pathology, transfusion medicine, genetics, immunology and molecular diagnostics. The reviews critically evaluate the status of current issues in the selected areas, with a focus on clinical laboratory diagnostics and latest advances. The adjective “critical” implies a balanced synthesis of results and conclusions that are frequently contradictory and controversial.
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