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IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-02-19 DOI:10.1007/s00604-025-07032-0

Linzhi Xie, Linxin He, Yang Fu, Yuxin Wei, Kun Zhang, Mei Chen

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引用次数: 0

摘要

利用支化铂金纳米球（B-Pt-Au NPs）作为标签和 MnO2-GO-Au 纳米片修饰电极，开发了一种新型电化学细胞传感器，采用三蛋白识别策略检测循环肿瘤细胞（CTC）。该系统集成了特异性磁分离、CTC 富集和灵敏的电化学检测功能，可识别全血中罕见的 A549 细胞。MnO2-GO-Au 纳米片以其高导电性和生物相容性而著称，被用来修饰裸电极，改善生物分子的电化学界面。只有当外周血中罕见的 A549 细胞的粘蛋白 1（MUC1）、上皮细胞粘附分子（EpCAM）和表皮生长因子受体（EGFR）这三种特定蛋白同时在细胞膜上表达时，B-Pt-Au NPs 才能接近传感界面，并通过其特定的电催化活性增强电流信号。所开发的细胞传感器的检测限低至 1 cells mL-1，更重要的是，它能准确区分 A549 细胞和其他癌细胞。因此，这种拟议的策略为复杂外周血中极其罕见的CTC的液体活检分析提供了一种强有力的工具，有望应用于非小细胞肺癌（NSCLC）的可靠早期诊断。图文摘要

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A three-site recognition cytosensor for accurate detection of circulating tumor cells based on novel branched Pt Au nanospheres and MnO2-GO-Au nanosheets

A novel electrochemical cytosensor was developed with a three-protein recognition strategy, employing branched Pt Au nanospheres (B-Pt-Au NPs) as tags and MnO₂-GO-Au nanosheet-modified electrodes for circulating tumor cells (CTC) detection. This system integrates specific magnetic separation and CTC enrichment with sensitive electrochemical detection to identify rare A549 cells in whole blood. MnO₂-GO-Au nanosheets, known for their high conductivity and biocompatibility, were used to modify a bare electrode, improving the electrochemical interface for biomolecules. The B-Pt-Au NPs could only approach the sensing interface and enhance the current signal through their specific electrocatalytic activity when all three specific proteins, i.e. mucin 1 (MUC1), epithelial cell adhesion molecule (EpCAM), and epidermal growth factor receptor (EGFR), of rare A549 cells in peripheral blood were simultaneously expressed on the cell membrane. The developed cytosensor demonstrated a detection limit as low as 1 cell mL⁻¹ and, more importantly, could accurately distinguish A549 cells from other cancer cells. Thus, this proposed strategy offers a powerful tool for liquid biopsy analysis of extremely rare CTCs in complex peripheral blood, with potential applications in reliable early diagnosis of non-small cell lung cancer (NSCLC).

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.