日本沼虾卵巢发育过程中CYP315 A1的分子特征及功能分析

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-02-20 DOI:10.1016/j.aqrep.2025.102700

Sufei Jiang , Wenyi Zhang , Jufeng Jiang , Mengying Zhang , Jisheng Wang , Yiwei Xiong , Hui Qiao , Hongtuo Fu

{"title":"日本沼虾卵巢发育过程中CYP315 A1的分子特征及功能分析","authors":"Sufei Jiang , Wenyi Zhang , Jufeng Jiang , Mengying Zhang , Jisheng Wang , Yiwei Xiong , Hui Qiao , Hongtuo Fu","doi":"10.1016/j.aqrep.2025.102700","DOIUrl":null,"url":null,"abstract":"<div><div>The CYP315A1 gene in Macrobrachium nipponense (named Mn-CYP315A1) was characterized from the “Insect hormone biosynthesis” signaling pathway in the hepatopancreas transcriptomes. The total cDNA length of Mn-CYP315A1 was 1582 bp, encoding 569 amino acids. It contained typical P450conserved domains. qPCR analysis indicated that this gene was highly expressed in the ovaries of adult females (P < 0.05), moderately expressed in their heart (P < 0.05), and minimally expressed in other tissues (P < 0.05). The expression of Mn-CYP315A1 was relatively stable during embryonic stages (CS to ZS) and from hatching to metamorphosis (L1 to L10) (P > 0.05), and increased significantly on the 10th day after metamorphosis (P < 0.05). Expression in mature ovaries increased sharply (P < 0.01), while it was relatively low in other stages (P < 0.05). In situ hybridization results showed that it was mainly expressed in the cytoplasmic membrane and nucleus of oocytes, and the signal was strongest during the mature stage (Ⅳ stage) and weakest during the non-development stage. The RNA interference results showed that the GSI and the cumulative proportion of ovary stage Ⅲ in the experimental group were significantly lower than those in the ds-GFP group after injection with CYP315A1 dsRNA (P < 0.05). The cumulative molting frequency of the ds-GFP group was about 2.5 times that of the ds-CYP315 A1 group. These results suggest that Mn-CYP315A1 has a dual role in promoting ovarian development and molting in female M. nipponense.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102700"},"PeriodicalIF":3.7000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular characterization and functional analysis of CYP315 A1 in ovarian development in Macrobrachium nipponense\",\"authors\":\"Sufei Jiang , Wenyi Zhang , Jufeng Jiang , Mengying Zhang , Jisheng Wang , Yiwei Xiong , Hui Qiao , Hongtuo Fu\",\"doi\":\"10.1016/j.aqrep.2025.102700\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The CYP315A1 gene in Macrobrachium nipponense (named Mn-CYP315A1) was characterized from the “Insect hormone biosynthesis” signaling pathway in the hepatopancreas transcriptomes. The total cDNA length of Mn-CYP315A1 was 1582 bp, encoding 569 amino acids. It contained typical P450conserved domains. qPCR analysis indicated that this gene was highly expressed in the ovaries of adult females (P < 0.05), moderately expressed in their heart (P < 0.05), and minimally expressed in other tissues (P < 0.05). The expression of Mn-CYP315A1 was relatively stable during embryonic stages (CS to ZS) and from hatching to metamorphosis (L1 to L10) (P > 0.05), and increased significantly on the 10th day after metamorphosis (P < 0.05). Expression in mature ovaries increased sharply (P < 0.01), while it was relatively low in other stages (P < 0.05). In situ hybridization results showed that it was mainly expressed in the cytoplasmic membrane and nucleus of oocytes, and the signal was strongest during the mature stage (Ⅳ stage) and weakest during the non-development stage. The RNA interference results showed that the GSI and the cumulative proportion of ovary stage Ⅲ in the experimental group were significantly lower than those in the ds-GFP group after injection with CYP315A1 dsRNA (P < 0.05). The cumulative molting frequency of the ds-GFP group was about 2.5 times that of the ds-CYP315 A1 group. These results suggest that Mn-CYP315A1 has a dual role in promoting ovarian development and molting in female M. nipponense.</div></div>\",\"PeriodicalId\":8103,\"journal\":{\"name\":\"Aquaculture Reports\",\"volume\":\"41 \",\"pages\":\"Article 102700\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-02-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture Reports\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352513425000869\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Reports","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352513425000869","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

摘要

日本沼虾（Macrobrachium nipponense） CYP315A1基因（命名为Mn-CYP315A1）从肝胰腺“昆虫激素生物合成”信号通路转录组中进行了表征。Mn-CYP315A1的cDNA总长度为1582 bp，编码569个氨基酸。它包含典型的p450保守结构域。qPCR分析显示，该基因在成年女性卵巢中高表达（P <； 0.05），在心脏中中表达（P <； 0.05），在其他组织中表达最低（P <； 0.05）。Mn-CYP315A1在胚胎期（CS ~ ZS）和孵化至变态期（L1 ~ L10）表达相对稳定（P >； 0.05），在变态后第10天表达显著升高（P <； 0.05）。成熟卵巢中表达量急剧升高（P <； 0.01），其他阶段表达量相对较低（P <； 0.05）。原位杂交结果显示，它主要表达于卵母细胞的细胞质膜和细胞核中，在成熟阶段（Ⅳ阶段）信号最强，在非发育阶段信号最弱。RNA干扰结果显示，注射CYP315A1 dsRNA后，实验组的GSI和卵巢分期累积比例Ⅲ均显著低于ds-GFP组（P <； 0.05）。ds-GFP组的累计蜕皮频率约为ds-CYP315 A1组的2.5倍。这些结果表明，Mn-CYP315A1在促进雌性日本绒猴卵巢发育和蜕皮中具有双重作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Molecular characterization and functional analysis of CYP315 A1 in ovarian development in Macrobrachium nipponense

The CYP315A1 gene in Macrobrachium nipponense (named Mn-CYP315A1) was characterized from the “Insect hormone biosynthesis” signaling pathway in the hepatopancreas transcriptomes. The total cDNA length of Mn-CYP315A1 was 1582 bp, encoding 569 amino acids. It contained typical P450conserved domains. qPCR analysis indicated that this gene was highly expressed in the ovaries of adult females (P < 0.05), moderately expressed in their heart (P < 0.05), and minimally expressed in other tissues (P < 0.05). The expression of Mn-CYP315A1 was relatively stable during embryonic stages (CS to ZS) and from hatching to metamorphosis (L1 to L10) (P > 0.05), and increased significantly on the 10th day after metamorphosis (P < 0.05). Expression in mature ovaries increased sharply (P < 0.01), while it was relatively low in other stages (P < 0.05). In situ hybridization results showed that it was mainly expressed in the cytoplasmic membrane and nucleus of oocytes, and the signal was strongest during the mature stage (Ⅳ stage) and weakest during the non-development stage. The RNA interference results showed that the GSI and the cumulative proportion of ovary stage Ⅲ in the experimental group were significantly lower than those in the ds-GFP group after injection with CYP315A1 dsRNA (P < 0.05). The cumulative molting frequency of the ds-GFP group was about 2.5 times that of the ds-CYP315 A1 group. These results suggest that Mn-CYP315A1 has a dual role in promoting ovarian development and molting in female M. nipponense.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.