从烟草根际土壤中分离的烟草链霉菌。

IF 2.1 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Antibiotics Pub Date : 2025-02-17 DOI:10.1038/s41429-024-00802-7

Kaiqin Li, Hao Zhang, Ying Qian, Ping Mo, Aihua Deng, Jian Gao, Wei Liang

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引用次数: 0

摘要

菌株HUAS MG91T是从常德市烟草根际土壤中分离到的一种新型放线菌。该菌株在高斯合成1号培养基上可形成短杆状孢子，表面光滑，孢子链可整流。16S rRNA基因序列比较表明，菌株HUAS MG91T与昆明链霉菌NBRC 14463 T的相似性最高（98.95%）。基于16S rRNA基因和基因组序列的系统发育分析表明，菌株HUAS MG91T与昆明S. s.k mingensis NBRC 14463 T亲缘关系较近。但两者间的ANIm和dDDH值分别为87.25%和28.60%，低于作为链霉菌种类划分指导值的96.7% ANIm阈值和70.0%临界值。根据与被试菌株的表型和基因型分化，提出菌株HUAS MG91T代表链霉菌属的新种，命名为tabacisoli Streptomyces sp. nov.，型菌株为HUAS MG91T （=MCCC 1K09288T = JCM 37027 T）。

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Streptomyces tabacisoli sp. nov, isolated from the rhizosphere soil of Nicotiana tabacum

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Streptomyces tabacisoli sp. nov, isolated from the rhizosphere soil of Nicotiana tabacum

Strain HUAS MG91T, a novel actinobacterium, was isolated from the rhizosphere soil of Nicotiana tabacum collected from Changde City, China. This strain could form short, rod-shaped spores with a smooth surface and Rectiflexibles spore chains on Gause’s synthetic No.1 medium. Comparison of 16S rRNA gene sequences showed that strain HUAS MG91T exhibited the highest similarity to Streptomyces kunmingensis NBRC 14463 T (98.95%). Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strain HUAS MG91T was closely related to S. kunmingensis NBRC 14463 T. However, the ANIm and dDDH values between them were 87.25% and 28.60%, respectively, lower than the 96.7% ANIm threshold and 70.0% cutoff point used as guideline values for Streptomyces species delineation. On the basis of phenotypic and genotypic differentiation from tested strains, strain HUAS MG91T is proposed to represent a novel species of the genus Streptomyces, named Streptomyces tabacisoli sp. nov. The type strain is HUAS MG91T (=MCCC 1K09288T = JCM 37027 T).

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来源期刊

Journal of Antibiotics 医学-免疫学

CiteScore

6.60

自引率

3.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Antibiotics seeks to promote research on antibiotics and related types of biologically active substances and publishes Articles, Review Articles, Brief Communication, Correspondence and other specially commissioned reports. The Journal of Antibiotics accepts papers on biochemical, chemical, microbiological and pharmacological studies. However, studies regarding human therapy do not fall under the journal’s scope. Contributions regarding recently discovered antibiotics and biologically active microbial products are particularly encouraged. Topics of particular interest within the journal''s scope include, but are not limited to, those listed below: Discovery of new antibiotics and related types of biologically active substances Production, isolation, characterization, structural elucidation, chemical synthesis and derivatization, biological activities, mechanisms of action, and structure-activity relationships of antibiotics and related types of biologically active substances Biosynthesis, bioconversion, taxonomy and genetic studies on producing microorganisms, as well as improvement of production of antibiotics and related types of biologically active substances Novel physical, chemical, biochemical, microbiological or pharmacological methods for detection, assay, determination, structural elucidation and evaluation of antibiotics and related types of biologically active substances Newly found properties, mechanisms of action and resistance-development of antibiotics and related types of biologically active substances.