牛膝草α-spinasterol通过NF-κB和Nrf2/HO-1通路改善炎症反应。

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-02-17 DOI:10.1038/s41598-025-90022-2

Qiongli Zeng, Weiting Xiao, Heng Zhang, Wei Liu, Xionglong Wang, Zhen Li, Yue Han, Zhi Wang, Shunxiang Li, Jinwei Yang, Wen Ouyang

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引用次数: 0

摘要

在前人研究的基础上，牛膝草（Achyranthes aspera L.）的低极性部分具有较强的抗炎活性。从芦笋低极性部位分离得到3个化合物，结构鉴定为α-spinasterol(1)、7,8-二氢spinasterol(2)、22,23-二氢spinasterol(3)。其中，α-spinasterol(1)在春冬季节含量较高，范围为0.0085 ~ 0.0157%。此外，在lps诱导的RAW264.7细胞炎症模型中，α-spinasterol显著降低IL-6、PGE2、TNF-α等细胞因子水平，抑制COX-2、5-LOX、p-IKKβ、p-NFκB、p-IkBα蛋白表达，促进Nrf2、HO-1、NQO1蛋白表达。因此，本研究表明α-spinasterol能够抑制lps诱导的RAW264.7细胞炎症，其机制可能与抑制NF-κB通路、激活Nrf2通路、减少炎症因子过度释放有关。

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α-spinasterol isolated from Achyranthes aspera L. ameliorates inflammation via NF-κB and Nrf2/HO-1 pathways.

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α-spinasterol isolated from Achyranthes aspera L. ameliorates inflammation via NF-κB and Nrf2/HO-1 pathways.

On the basis of previous studies, the low-polar part of Achyranthes aspera L. (A. aspera) had strong anti-inflammatory activity. Three compounds were isolated from the low polarity fraction of A. aspera, and their structures were identified as α-spinasterol (1), 7,8-dihydrospinasterol (2), 22,23-dihydrospinasterol (3). Among them, the content of α-spinasterol (1) in A. aspera was higher in the spring and winter seasons through HPLC methods, ranging from 0.0085 to 0.0157%. Futhermore, in the LPS-induced RAW264.7 cells inflammation model, α-spinasterol significantly reduced the levels of cytokines such as IL-6, PGE2 and TNF-α, inhibited the expression of COX-2, 5-LOX, p-IKKβ, p-NFκB and p-IkBα proteins, and promoted the expression of Nrf2, HO-1 and NQO1 proteins. Therefore, this study showed that α-spinasterol can inhibit LPS-induced RAW264.7 cells inflammation, and its mechanism may be related to the inhibition of NF-κB pathway, activation of Nrf2 pathway, and reduction of excessive release of inflammatory factors.

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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