利用CRISPR/Cas9系统构建敲除camd8基因的纯合子人胚胎干细胞系WAe009-A-2R

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-02-15 DOI:10.1016/j.scr.2025.103683

Wenmin Pan , Yuhe Yang , Shun Zhang , Xiaoyu Liu , Huijuan Hu , Jia Qu , Hui Liu

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引用次数: 0

摘要

camd8是A2M/C3 （α-2-巨球蛋白/补体3）蛋白家族的一员，在人类胎儿晶状体和远端神经视网膜中显著表达。camd8基因突变与前段发育不良和原发性先天性青光眼有关。我们利用CRISPR/Cas9技术建立了敲除camd8的纯合子人胚胎干细胞系，用于视网膜类器官的分化，目的是探索camd8在人眼早期发育中的作用。CPAMD8敲除细胞系表现出正常的形态、多能性和核型，为研究camd8在眼科中的功能提供了有价值的研究工具。

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Generation of a homozygous CPAMD8 knockout human embryonic stem cell line (WAe009-A-2R) by CRISPR/Cas9 system

CPAMD8, a constituent of the A2M/C3 (α-2-macroglobulin/complement 3) protein family, is strikingly expressed in the human fetal lens and distal neural retina. Mutations in CPAMD8 have been linked to anterior segment dysgenesis and primary congenital glaucoma. We utilized CRISPR/Cas9 technology to establish a homozygous CPAMD8 knockout human embryonic stem cell line for differentiating retinal organoids, with the intent of exploring the role of CPAMD8 in the early development of the human eye. The CPAMD8 knockout cell line exhibits normal morphology, pluripotency, and karyotype, serving as a valuable research tool for investigating the functions of CPAMD8 in ophthalmology.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.