间充质干细胞与间充质干细胞衍生的外泌体在糖尿病模型中作为潜在的自噬通路调节剂。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences Pub Date : 2025-02-14 DOI:10.1016/j.advms.2025.02.004

Fatma Y. Meligy , Hanan Sharaf El-Deen Mohammed , Amal T. Abou Elghait , Heba K. Mohamed , Israa El-Sayed Mohamed Ashry , Ayat Abdel-Rahman Sayed , Ola A. Hussein , Ahmed Salman , Tarek Atia , Abir S. Mohamed , Nour H. Behnsawy , Safy Salah Gaber , Hader I. Sakr , Salwa Fares Ahmed

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引用次数: 0

摘要

目的：本研究比较了骨髓间充质干细胞（BM-MSCs）和BM-MSCs来源的外泌体对链脲佐菌素（STZ）诱导的糖尿病大鼠自噬受损的潜在作用。材料与方法：注射STZ后3 d，给予单剂量（3 × 10 × 6） BM-MSCs或BM-MSCs来源的外泌体（80 μg/大鼠），评估其对非糖尿病和糖尿病对照大鼠的作用。我们通过光镜和电镜评估胰腺结构，并评估其胰岛素和自噬标记物P62的免疫组织化学染色。采用PCR检测自噬标志物LC3基因的表达。结果：BM-MSCs和BM-MSCs衍生的外泌体均表现出胰腺组织的组织学恢复。两种处理均显著提高胰岛素水平，显著降低自噬标志物P62和LC3。结论：我们的研究结果表明，BM-MSCs和BM-MSCs衍生的外泌体都为调节糖尿病提供了一种潜在的选择。

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Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model

Purpose

This work compared the potential effects of bone marrow mesenchymal stem cells (BM-MSCs) with BM-MSCs-derived exosomes against impaired autophagy in streptozotocin (STZ)-induced diabetic rats.

Materials and methods

Three days after STZ injection, a single dose of (3 × 10^6) BM- MSCs or BM-MSCs-derived exosomes (80 μg/rat) was administered to evaluate their effects against nondiabetic and diabetic control rats. We assessed pancreatic structure via light and electron microscopy and evaluated its staining for insulin and the autophagy marker P62 immunohistochemically. Moreover, autophagy marker LC3 gene expression was examined by PCR.

Results

Both BM-MSCs and BM-MSCs derived exosomes showed histological restoration of pancreatic tissues. Both treatments markedly increased the amount of insulin and significantly decreased the autophagy markers P62 and LC3.

Conclusion

Our findings suggest that both BM-MSCs and BM-MSCs-derived exosomes provides a potential alternative to modulate diabetes mellitus.

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来源期刊

Advances in medical sciences 医学-医学：研究与实验

CiteScore

5.00

自引率

0.00%

发文量

审稿时长

25 days

期刊介绍： Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.