封面特写：条件分裂蛋白重组生产环肽Kalata B1 （ChemBioChem 4/2025）

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

ChemBioChem Pub Date : 2025-02-18 DOI:10.1002/cbic.202580404

Dr. Abdulkadir Yayci, Dr. Yen-Hua Huang, Dr. Peta J. Harvey, Prof. David J. Craik

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引用次数: 0

摘要

该图展示了为大规模生产从重组表达的分裂意图的环核苷酸而设计的工作流程的可扩展性和操作便利性。前体蛋白的转化很容易从大肠杆菌中产生和纯化，只需将分裂的蛋白的两个片段混合就可以产生所需的环状肽。更多信息可以在10.1002/cbic文章中找到。[202400591]作者：David J. Craik及其同事。封面由Dr Peta J. Harvey设计。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Cover Feature: Recombinant Production of The Cyclotide Kalata B1 by Conditional Split Inteins (ChemBioChem 4/2025)

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Cover Feature: Recombinant Production of The Cyclotide Kalata B1 by Conditional Split Inteins (ChemBioChem 4/2025)

This illustration represents the facile scalability and ease of operation of a workflow designed for the mass production of cyclotides from recombinantly expressed split inteins. Conversion of precursor proteins, easily produced and purified from E. coli, proceeds by simply mixing two fragments of a split intein to yield the desired cyclic peptide. More information can be found in the article 10.1002/cbic.202400591 by David J. Craik and co-workers. Cover art designed by Dr Peta J. Harvey.

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来源期刊

ChemBioChem 生物-生化与分子生物学

CiteScore

6.10

自引率

3.10%

发文量

407

审稿时长

1 months

期刊介绍： ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).