限时喂养对高脂肪饮食和含糖饮料诱导的肥胖前驱糖尿病大鼠模型的血糖指数、血管氧化应激和炎症的影响

IF 3.2 3区医学 Q2 NUTRITION & DIETETICS

Nutrition Pub Date : 2025-01-23 DOI:10.1016/j.nut.2025.112692

Siti Qusyasyiah Ahmad Suhaimi , Ahmad Khusairi Azemi , Siti Safiah Mokhtar , Anani Aila Mat Zin , Aida Hanum Ghulam Rasool

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引用次数: 0

摘要

目的探讨限时喂养（TRF）对肥胖前驱糖尿病模型大鼠血糖指标及主动脉组织氧化应激和炎症的影响。方法与方法雄性sd大鼠分为正常组和肥胖组。高脂饮食加蔗糖水（HFSD） 10周诱导肥胖前驱糖尿病；给正常大鼠标准饮食和白开水。在接下来的6周内，将大鼠分为正常组（NR），继续进行标准饮食；正常组改为TRF +标准日粮（NR + TRFSD）；前驱糖尿病组（OR）继续HFSD治疗；前驱糖尿病组改为TRFHFSD组（OR + TRFHFSD组）；前驱糖尿病组改为标准饮食中的TRF (OR + TRFSD)；前驱糖尿病组改为标准饮食（OR + SD）。然后处死大鼠，分离主动脉组织，定量检测氧化应激标志物丙二醛、抗氧化酶超氧化物歧化酶、炎症标志物肿瘤坏死因子-α和白细胞介素1。同时测量空腹血糖（FBG）、体重、Lee's指数、血清胰岛素水平和胰岛素抵抗（胰岛素抵抗的稳态模型评估）。结果HFDSD治疗10周后，肥胖组的平均FBG和体重均高于正常组。两组接受TRF治疗的肥胖糖尿病前期患者的肿瘤坏死因子-α、白细胞介素1、体重、李氏指数、空腹血糖和胰岛素抵抗水平均有所降低。此外，肥胖前驱糖尿病TRF合并SD还降低了脂质过氧化（丙二醛）水平、胰岛素水平和抗氧化酶（超氧化物歧化酶）水平。结论trf可减轻肥胖糖尿病前期大鼠体重，改善血糖指标、血管氧化应激和炎症反应。

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Impact of time-restricted feeding on glycemic indices, vascular oxidative stress, and inflammation in an obese prediabetes rat model induced by a high-fat diet and sugar drink

Objective

This study aims to investigate the effects of time-restricted feeding (TRF) on glycaemic indices and aortic tissue oxidative stress and inflammation in an obese prediabetes rat model.

Methods and Procedures

Male Sprague-Dawley rats were divided into two normal and four obese groups. Obese prediabetes was induced by feeding a high-fat diet and sucrose water (HFSD) for 10 weeks; normal rats were given a standard diet and plain water. For the next 6 weeks, rats were grouped into the normal group (NR), which continued on the standard diet; the normal group was switched to TRF with the standard diet (NR + TRFSD); the prediabetes group (OR) was continued on HFSD; the prediabetes group was switched to TRF of HFSD (OR + TRFHFSD); the prediabetes group was switched to TRF of the standard diet (OR + TRFSD); and the prediabetes group was switched to the standard diet (OR + SD). Rats were then sacrificed, and aortic tissues were isolated and quantified for oxidative stress markers malondialdehyde, antioxidant enzyme superoxide dismutase, and inflammation markers tumor necrosis factor-α, and interleukin 1. Fasting blood glucose (FBG), body weight, Lee's index, serum insulin level, and resistance (Homeostatic Model Assessment of Insulin Resistance) were also measured.

Results

Mean FBG and body weight in obese groups were higher compared to the normal groups after 10 weeks of HFDSD. Both obese-prediabetes groups that underwent TRF had reduced levels of tumor necrosis factor-α, interleukin 1, body weight, Lee's index, FBG, and insulin resistance. Furthermore, obese prediabetes on TRF with SD also reduced levels of lipid peroxidation (malondialdehyde), insulin levels and increased levels of the antioxidant enzyme (superoxide dismutase).

Conclusion

TRF reduced weight, improved glycaemic indices, vascular oxidative stress, and inflammation in obese-prediabetic rats.

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来源期刊

Nutrition 医学-营养学

CiteScore

7.80

自引率

2.30%

发文量

300

审稿时长

60 days

期刊介绍： Nutrition has an open access mirror journal Nutrition: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Founded by Michael M. Meguid in the early 1980''s, Nutrition presents advances in nutrition research and science, informs its readers on new and advancing technologies and data in clinical nutrition practice, encourages the application of outcomes research and meta-analyses to problems in patient-related nutrition; and seeks to help clarify and set the research, policy and practice agenda for nutrition science to enhance human well-being in the years ahead.