内生真菌黑孢菌的稀有氯化真菌代谢物和α -吡咯酮。

IF 4.3 3区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Chika C. Abba, Peter M. Eze*, Sherif S. Ebada*, Nchekwube K. Eze, Peter Proksch, Nicole Teusch, Festus B. C. Okoye and Chukwueweniwe J. Eboka, 
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引用次数: 0

摘要

内生微生物已被认为是新的化学实体的潜在来源,在制药,生物技术,农业和其他行业的应用。本研究研究了从尼日利亚植物Gongronema latifolium中分离的内生黑孢菌sp.产生的次生代谢物。按照标准程序进行真菌分离、分类鉴定、发酵和提取次生代谢物。采用1,1-二苯基-2-苦味酰肼(DPPH)抗氧化法和琼脂孔扩散法测定真菌提取物的抗氧化和抗菌性能。各种色谱和光谱技术被用于分离、纯化和表征真菌提取物中的化合物。在500 μg/mL浓度下,真菌粗提物抗氧化活性平均,抑制率为48%。提取物对枯草芽孢杆菌和伤寒沙门氏菌的抑菌活性为1 mg/mL,抑菌带直径均为2 mm。真菌提取物产生了六种化合物,包括罕见的氯化代谢物acrodontiolamide(1)和五种α-吡酮衍生物:hydroxypestalopyrone(2)、pestalopyrone(3)、pestalotiopyrone D(4)、顺式-4-hydroxymellein(5)及其反式异构体(6)。有趣的是,这是继1993年首次报道后,自然界中第二篇关于acrodontiolamide(1)的报道。这些化合物具有广泛的已知生物活性,包括抗菌、抗肿瘤和细胞毒性作用,在药物开发中具有很大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rare Chlorinated Fungal Metabolite and Alpha-Pyrones from an Endophytic Fungus Nigrospora sp.

Endophytic microorganisms have been recognized as potential sources of new chemical entities with applications in the pharmaceutical, biotechnology, agricultural, and other industries. This study investigated the secondary metabolites produced by an endophytic Nigrospora sp. isolated from the Nigerian plant, Gongronema latifolium. Standard procedures were followed for fungal isolation, taxonomic identification, fermentation, and extraction of secondary metabolites. The antioxidant and antimicrobial properties of the fungal extract were assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) antioxidant assay and the agar-well diffusion assay, respectively. Various chromatographic and spectroscopic techniques were used to isolate, purify, and characterize compounds from the fungal extract. At 500 μg/mL, the fungal crude extract showed average antioxidant activity with a 48% inhibition. The extract also demonstrated moderate antimicrobial activity at 1 mg/mL against Bacillus subtilis and Salmonella typhi, with an inhibition zone diameter of 2 mm produced against both test strains. The fungal extract yielded six compounds, including the rare, chlorinated metabolite, acrodontiolamide (1), and five α-pyrone derivatives: hydroxypestalopyrone (2), pestalopyrone (3), pestalotiopyrone D (4), cis-4-hydroxymellein (5), and its trans-isomer (6). Interestingly, this is the second report of acrodontiolamide (1) in nature, after its first report in 1993. These compounds possess a wide range of known biological activities, including antimicrobial, antitumor, and cytotoxic effects, valorizing their potential in drug development.

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来源期刊
ACS Omega
ACS Omega Chemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍: ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.
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