miRNA-144-5p调控自噬对患有 "肌少症 "的老年人的影响及其机制

IF 5.2 2区 医学 Q1 GERIATRICS & GERONTOLOGY
Mengdie Hu, Ying Zhang, Hong Ding, Rui Chao, Zhidong Cao
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引用次数: 0

摘要

背景:由于细胞自噬的减少或细胞自噬过程的紊乱,衰老不可避免地引发过多的细胞凋亡。这反过来又会导致肌肉蛋白质的加速分解,从而加剧骨骼肌的持续退化,并加剧老年性肌肉减少症的严重程度。本研究旨在探讨mirna调控的自噬在老年性肌少症中的作用及机制。方法:筛选与肌少症相关的mirna,预测显著mirna的靶基因。体内外实验验证了显著差异表达的miRNA-144-5p对细胞衰老和自噬的影响。结果:抑制miR-144-5p可增强小鼠成肌细胞的增殖,增加MHC及自噬标志物LC3II/LC3I、Beclin-1的表达,促进小鼠成肌细胞自噬体的形成,减少小鼠成肌细胞衰老细胞数量及衰老相关蛋白乙酰化p53、p53、p21的表达。miR-144-5p通过调节下游靶基因Atg2A影响成肌细胞衰老、成肌分化和自噬。抑制miR-144-5p显著增加老年小鼠后肢握力,老年小鼠和幼龄小鼠的CSA也显著增加。结论:各项实验均表明miRNA-144-5p对自噬的调控及老年性肌少症的发生有显著影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect and mechanism of miRNA-144-5p-regulated autophagy in older adults with Sarcopenia.

Background: Advanced aging invariably triggers an overabundance of apoptosis, stemming from diminished autophagy or a disarray in cellular autophagic processes. This, in turn, leads to an accelerated breakdown of muscle proteins, which exacerbates the ongoing deterioration of skeletal muscle and intensifies the severity of senile sarcopenia. This study aimed to investigate the role and mechanism of miRNA-regulated autophagy in senile sarcopenia.

Methods: The miRNAs associated with sarcopenia were screened, and the target genes of significant miRNAs were predicted. The effects of significantly differentially expressed miRNA-144-5p on cell aging and autophagy were validated in vivo and in vitro.

Results: The inhibition of miR-144-5p enhanced the multiplication of mouse myoblasts, increased the expression of MHC and autophagic markers LC3II/LC3I and Beclin-1, facilitated the formation of autophagosomes in mouse myoblasts, and reduced the number of aging cells and the expression of senescence-related proteins acetylated p53, p53, and p21 expression in mouse myoblasts. miR-144-5p affects myoblast senescence, myogenic differentiation, and autophagy by regulating the downstream target gene, Atg2A. Inhibiting miR-144-5p markedly increased the grip strength of the posterior limb in old mice, and the CSA of old mice and young mice was also markedly increased.

Conclusion: All experiments have demonstrated that miRNA-144-5p has a significant impact on the regulation of autophagy and the development of senile sarcopenia.

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来源期刊
Immunity & Ageing
Immunity & Ageing GERIATRICS & GERONTOLOGY-IMMUNOLOGY
CiteScore
10.20
自引率
3.80%
发文量
55
期刊介绍: Immunity & Ageing is a specialist open access journal that was first published in 2004. The journal focuses on the impact of ageing on immune systems, the influence of aged immune systems on organismal well-being and longevity, age-associated diseases with immune etiology, and potential immune interventions to increase health span. All articles published in Immunity & Ageing are indexed in the following databases: Biological Abstracts, BIOSIS, CAS, Citebase, DOAJ, Embase, Google Scholar, Journal Citation Reports/Science Edition, OAIster, PubMed, PubMed Central, Science Citation Index Expanded, SCImago, Scopus, SOCOLAR, and Zetoc.
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