正常视力的I型神经纤维瘤病小鼠模型视网膜功能和结构的性别依赖性变化。

IF 3 2区 医学 Q1 OPHTHALMOLOGY
Francisco M. Ribeiro , Joana Gonçalves , Luís Coelho , Miguel Castelo-Branco , João Martins
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引用次数: 0

摘要

我们的目的是表征1型神经纤维瘤病(NF1)小鼠模型的早期视觉系统的结构和功能,这是自闭症谱系障碍(ASD)的综合征模型。我们使用Nf1+/-小鼠和WT幼崽,通过光学相干断层扫描(OCT)进行视网膜结构分析,并通过电生理记录进行功能评估。然后,我们使用视动反应(OMR)和对视觉刺激熟悉度的敏感性进行了行为视觉测试。从结构上分析,我们发现雄性Nf1+/-小鼠的神经节细胞层-内丛状层(GCL-IPL)和外核层(ONL)厚度较WT仔鼠增加。在视网膜电生理方面,雌性Nf1+/-小鼠的第二振荡电位(OP2)幅度与WT幼崽相比有所增加。然而,Nf1+/-和WT小鼠均表现出正常的视敏度,并能表现出规律的视觉刺激熟悉性反应。虽然结构性的性别依赖性变化与先前的脑解剖学测量结果一致,但振荡活动的微妙性别依赖性变化可能与NF1中发现的gaba能神经传递变化有关。总的来说,这些结构和功能的变化似乎并没有转化为视觉行为的改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sex-dependent variations of retinal function and architecture in a neurofibromatosis type I mouse model with normal vision
We aimed to characterize the structure and function of the early visual system of the neurofibromatosis type 1 (NF1) mouse model, a syndromic model of autism spectrum disorders (ASD).
We used Nf1+/− mice and WT littermates and performed retinal structural analysis by optical coherence tomography (OCT), and functional assessment by electrophysiological recordings. We then performed behavioral visual tests using optomotor response (OMR) and sensitivity to visual stimulus familiarity.
From the structural analysis, we found increased thickness for ganglion cell layer-inner plexiform layer (GCL-IPL) and outer nuclear layer (ONL) in male Nf1+/− mice compared with WT littermates. Regarding retinal electrophysiology, female Nf1+/− mice exhibited increased amplitudes for the second oscillatory potential (OP2) compared with WT littermates. Nevertheless, both Nf1+/− and WT mice presented normal visual acuity as measured by OMR and were able to exhibit regular visual stimulus familiarity responses.
While structural sex-dependent changes are in line with previous results for brain anatomic measures, the subtle sex-dependent changes in oscillatory activity may relate to GABAergic neurotransmission changes found in NF1. Overall, these structural and functional changes do not seem to translate into visual behavioral alterations.
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来源期刊
Experimental eye research
Experimental eye research 医学-眼科学
CiteScore
6.80
自引率
5.90%
发文量
323
审稿时长
66 days
期刊介绍: The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.
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