在H1 (WA01) hESC背景下CNPY3敲除细胞系的产生

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-02-11 DOI:10.1016/j.scr.2025.103676

Xing Qi , Feng Yao , Shan Yongli , Zhong Xiaofen

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引用次数: 0

摘要

CNPY3基因编码一种蛋白质，该蛋白质与toll样受体（TLR）蛋白家族成员相互作用，并作为伴侣，帮助这些蛋白质正确折叠和输出。我们利用CRISPR/Cas9基因组编辑技术，构建了纯合子敲除CNPY3的人胚胎干细胞（hESC）细胞系WAe001-A-2T （H1-CNPY3−/−）。WAe001-A-2T细胞系核型正常，保持了未分化hESCs的典型特征，包括多能性基因表达和体内分化潜力。CNPY3敲除细胞系为研究CNPY3基因在体外胚胎发育和谱系分化中的作用提供了宝贵的资源。

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Generation of CNPY3 knock out cell line in the H1 (WA01) hESC background

The CNPY3 gene encodes a protein that interacts with members of the toll-like receptor (TLR) protein family and functions as a chaperone, aiding in the proper folding and export of these proteins. We generated a homozygous CNPY3 knockout human embryonic stem cell (hESC) line WAe001-A-2T (H1-CNPY3^−/−), using CRISPR/Cas9 genome editing technology. The WAe001-A-2T cell line exhibited a normal karyotype and maintained the typical characteristics of undifferentiated hESCs, including pluripotent gene expression and differentiation potential in vivo. The CNPY3 knockout cell line serves as a valuable resource for investigating the role of the CNPY3 gene in embryonic development and lineage differentiation in vitro.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.