水稻病毒抗性策略的革命：从RNAi到精确基因组编辑的进化

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-02-10 DOI:10.1016/j.virol.2025.110449

Gaurav Kumar, Indranil Dasgupta

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引用次数: 0

摘要

水稻病毒是对全球粮食安全的重大威胁，在主要水稻种植区造成重大产量损失。RNA干扰（RNAi）通过沉默必需的病毒基因在水稻病毒抗性工程中起着至关重要的作用。然而，基因组编辑的出现，特别是CRISPR/Cas的出现，通过允许精确改变病毒易感基因，提供更持久和更有针对性的抗性，改变了这一领域。本文综述了RNAi策略的进展和向CRISPR/Cas技术的转变，强调了基因组编辑如何解决RNAi的局限性，例如更广泛的病毒株覆盖和更强的抗性。这些工具与先进的育种方法相结合，有望开发出具有持久、广谱抗病毒能力的水稻品种，为可持续的水稻生产和粮食安全做出贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Revolutionizing viral resistance strategies in rice: Evolution from RNAi to precision genome editing

Rice viruses are a major threat to global food security, causing significant yield losses in key rice growing regions. RNA interference (RNAi) has been crucial in engineering viral resistance in rice by silencing essential viral genes. However, the advent of genome editing, especially CRISPR/Cas, has transformed this field by allowing precise alterations of viral susceptibility genes, offering more durable and targeted resistance. This review examines the advances in RNAi strategies and the shift toward CRISPR/Cas technologies, highlighting how genome editing addresses RNAi's limitations, such as broader viral strain coverage and stronger resistance. These tools, integrated with advanced breeding methods, promise to develop rice varieties with durable, broad-spectrum virus resistance, contributing to sustainable rice production and food security.

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.