四川和重庆番茄萎黄病毒分离株的全基因组分析

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-02-11 DOI:10.1016/j.virol.2025.110447

Xiaolong Yang, Jie Li, Mingrui Gao, Puxin Huang, Mingjun Li, Gentu Wu, Ling Qing

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引用次数: 0

摘要

番茄褪绿病毒（ToCV）最近成为中国作物生产的重大威胁。在这项研究中，我们收集了来自四川和重庆的番茄叶片样本，显示出脉间褪绿、卷曲和变黄的症状。逆转录聚合酶链反应（RT-PCR）检测结果显示，四川省10份样本中有3份感染ToCV，重庆市24份样本中有9份感染ToCV。利用侵染叶片（SC744和CQ630）的cDNA组装出ToCV的全基因组序列，并将其存入GenBank，登录号为CQ630: PQ153240和PQ153241，以及SC744: PQ261056和PQ261057。系统发育分析表明，四川和重庆的ToCV分离株与bj ToCV分离株亲缘关系较近。序列恒等矩阵表明，p22变异率最高，为94.3% ~ 98.4%。P6是最保守的，恒等式在98%到100%之间。在RNA2中，p4在93.9% - 100%之间变化，而Hsp70在97.9%-99%之间保持高度保守。本研究首次报道了四川和重庆地区ToCV的全基因组序列，提高了我们对ToCV在中国的分布和遗传多样性的认识。

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Complete genomic analysis of tomato chlorosis virus isolates from Sichuan and Chongqing, China

Tomato chlorosis virus (ToCV) has recently emerged as a significant threat to crop production in China. In this study, we collected tomato leaf samples displaying symptoms of interveinal chlorosis, curling, and yellowing from Sichuan Province and Chongqing City. Reverse transcription polymerase chain reaction (RT-PCR) analysis confirmed that 3 out of 10 samples from Sichuan and 9 out of 24 samples from Chongqing were infected with ToCV. Complete genomic sequences of ToCV were assembled from the cDNA of two infected leaves (SC744 and CQ630), which were deposited in GenBank under accession numbers CQ630: PQ153240 and PQ153241 and SC744: PQ261056 and PQ261057. Phylogenetic analysis indicated that ToCV isolates from the Sichuan Province and Chongqing City were closely related to the ToCV-BJ isolate. The sequence identity matrix shows that p22 has the highest variability at 94.3%–98.4%. p6 is the most conserved, with identities between 98% and 100%. In RNA2, p4 varies from 93.9% to 100%, while Hsp70 remains highly conserved at 97.9%–99%. This study provides the first report on the complete genomic sequences of ToCV from Sichuan Province and Chongqing City, enhancing our understanding of ToCV distribution and genetic diversity in China.

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.