Antibacterial mechanism of lysine against Escherichia coli O157:H7, its action on lettuce and in reducing the severity of murine colitis

The aim of the present study was to evaluate the mechanism of antimicrobial action of Lysine (Lys) against Escherichia coli (E. coli) O157:H7. Lys alters the permeability and morphology of bacterial cell membranes, leading to leakage of intracellular material. Using agarose gel electrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE), it was found that high concentrations of Lys disrupted the DNA and protein primary structure of E. coli O157:H7. Lys also inhibited the ability of E. coli biofilm formation in a concentration-dependent manner. Subinhibitory concentration of Lys reduces the swimming and swarming ability of E. coli O157:H7 and its ability to adhere and to invade Caco-2 cells. The use of Lys on lettuce inoculated with E. coli O157:H7 showed good disinfection efficiency. Further studies revealed that Lys had both preventive and therapeutic effects on the severity of colitis induced by E. coli O157:H7 infected mice, and that the preventive effect was greater than the therapeutic effect.