早期开角型青光眼中adamts10突变比格犬房水中促炎细胞因子的研究

IF 1.7 4区农林科学 Q2 VETERINARY SCIENCES

Veterinary ophthalmology Pub Date : 2025-02-13 DOI:10.1111/vop.13321

Elisabeth Collins, Christine D Harman, Amanda L Jacobson, Carolina Mehaffy, Kim R Love, András M Komáromy, Michala de Linde Henriksen

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引用次数: 0

摘要

目的：探讨小猎犬adamts10突变体早期开角型青光眼（OAG）房水中促炎细胞因子的水平。研究动物：24只小猎犬被纳入这项病例对照研究。本研究招募了三组adamts10突变型研究犬：(1)眼内压（IOP） 15-25 mmHg（正常IOP）， (2) IOP 25-30 mmHg, (3) IOP 30-36 mmHg。一个未受影响的对照组是adamts10野生型（WT）或杂合子载体，具有正常的IOPs。方法：收集房水样品，使用犬细胞因子SpikeMix和多反应监测（MRM-MS）靶质谱分析16种促炎细胞因子。采用Kruskal-Wallis检验和Spearman’s rho （ρ）相关分析四组间促炎因子的统计学差异以及促炎因子与IOPs的相关性。结果：IOP 15-25 mmHg (n = 8)；眼压25-30 mmHg (n = 6)；IOP 30-36 mmHg (n = 5)；对照组（WT n = 3，载体n = 2，总n = 5）。四组间促炎细胞因子差异无统计学意义（p值均为0.05）。IL-13与IOP有中度正相关，但无统计学意义（ρ = 0.373, p = 0.073）；IL-1β与IOP有中度负相关，但也无显著性差异（ρ = -0.344, p = 0.100）。其他促炎因子与IOPs均有轻度相关性（|ρ| 0.05）。结论：犬ADAMTS10-OAG的促炎细胞因子未因IOP升高而发生显著变化。

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Pro-Inflammatory Cytokines in Aqueous Humor From ADAMTS10-Mutant Beagles at an Early Stage of Open-Angle Glaucoma.

Objective: To investigate the level of pro-inflammatory cytokines in aqueous humor from ADAMTS10-mutant research Beagles at an early stage of open-angle glaucoma (OAG).

Animals studied: Twenty-four research Beagles were enrolled into this case-controlled study. Three groups of ADAMTS10-mutant research Beagles were enrolled in this study: (1) Intraocular pressure (IOP) 15-25 mmHg (normotensive IOP), (2) IOP 25-30 mmHg, and (3) IOP 30-36 mmHg. An unaffected control group of Beagles that were either ADAMTS10-wildtype (WT) or heterozygote-carrier with normotensive IOPs were also enrolled.

Procedures: Aqueous humor samples were collected and analyzed for 16 pro-inflammatory cytokines using the Canine Cytokine SpikeMix and target mass spectrometry via multiple reaction monitoring (MRM-MS). Statistical differences between the four groups' pro-inflammatory cytokines, as well as correlations between pro-inflammatory cytokines and IOPs, were analyzed using Kruskal-Wallis tests and Spearman's rho (ρ) correlations, respectively.

Results: IOP 15-25 mmHg (n = 8); IOP 25-30 mmHg (n = 6); IOP 30-36 mmHg (n = 5); and Control (WT n = 3, carrier n = 2, total n = 5). There were no differences between pro-inflammatory cytokines across the four groups (all p-values > 0.05). IL-13 had a moderate positive correlation with IOP, but was non-significant (ρ = 0.373, p = 0.073); IL-1β had a moderate negative correlation with IOP but was also non-significant (ρ = -0.344, p = 0.100). All other pro-inflammatory cytokines had only mild correlation with IOPs (|ρ| < 0.229, p > 0.05).

Conclusions: There were no significant changes in the investigated pro-inflammatory cytokines with elevated IOP in canine ADAMTS10-OAG.

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来源期刊

Veterinary ophthalmology 农林科学-兽医学

CiteScore

2.70

自引率

37.50%

发文量

审稿时长

12-24 weeks

期刊介绍： Veterinary Ophthalmology is a peer-reviewed, international journal that welcomes submission of manuscripts directed towards academic researchers of veterinary ophthalmology, specialists and general practitioners with a strong ophthalmology interest. Articles include those relating to all aspects of: Clinical and investigational veterinary and comparative ophthalmology; Prospective and retrospective studies or reviews of naturally occurring ocular disease in veterinary species; Experimental models of both animal and human ocular disease in veterinary species; Anatomic studies of the animal eye; Physiological studies of the animal eye; Pharmacological studies of the animal eye.