富血浆纤维蛋白凝胶与脂肪来源的异体间充质干细胞:治疗二度深度烧伤创面的新方法表征及体内研究。

IF 1.4 4区 医学 Q4 CELL BIOLOGY
Kimia Didehvar, Najmeh Kamali, Mehdi Haghshenas, Reyhaneh Yarmohammadi, Ghazaleh Larijani, Seyedeh Lena Mohebbi, Mohammad Amir Amirkhani, Naser Amini
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引用次数: 0

摘要

用于紧急情况的生物相容性和易于获得的伤口敷料已被证明更具成本效益,同时也降低了免疫系统介导反应的风险。在这个项目中,我们研究了使用新鲜血液来源的基质作为伤口敷料,基于3D药物负载的富血浆纤维蛋白(PRF)支架,支持自体干细胞移植以再生烧伤造成的皮肤组织。以静脉血制备PRF支架,并从大鼠内脏脂肪组织中分离脂肪组织源性干细胞(ADSCs)。体外分析后,将PRF凝胶和ADSCs移植到大鼠背部二度深度烧伤创面。在第5、10、15和21天进行组织病理学分析和伤口大小测量。研究结果显示,PRF凝胶作为一种细胞相容性支架,具有抗菌药物释放的潜力(持续时间长达3天,释放率高达89.7%),显著促进了治疗组的愈合过程。在第15天,prf处理组的组织病理学观察到伤口大小缩小,皮肤细胞成熟,组织良好,胶原纤维更厚,总体组织病理学参数平均得分为(2.83±0.04)(满分为3分)。支架组创面收缩最大(5.32±0.61 mm2),对照组创面收缩最大(7.96±0.82 mm2) (p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Plasma-rich fibrin gel and adipose-derived allogeneic mesenchymal stem cells: innovation in the treatment of second-degree deep burn wound; characterization and in-vivo study.

A biocompatible and readily available wound dressing for emergencies has been shown to be more cost-effective, while also reducing the risk of immune system-mediated reactions. In this project, we investigated the use of a fresh blood-derived matrix as a wound dressing, based on a 3D drug-loaded Plasma-rich Fibrin (PRF) scaffold, to support the transplantation of autologous stem cells for regenerating skin tissues lost due to burns. PRF scaffold was prepared from venous blood, and adipose tissue-derived stem cells (ADSCs) were isolated from the visceral fat tissue of rats. Following in vitro analysis, PRF gel and ADSCs were transplanted onto second-degree deep burn wounds on the backs of rats. Histopathological analysis and wound size measurements were conducted on days 5, 10, 15, and 21. The findings revealed that PRF gel, as a cyto-compatible scaffold with the potential for antibacterial drug release (sustained for up to 3 days, with up to 89.7% release), significantly enhanced the healing process in the treatment group. On day 15, a reduced wound size, mature skin cells, and well-organized, thicker collagen fibers were observed in the histopathology of the PRF-treated groups, which scored an average of (2.83  ±  0.04) out of 3 for overall histopathological parameters. The greatest wound contraction was seen in the scaffold-treated groups (5.32  ±   0.61 mm2), compared with the control group (7.96 ±  0.82 mm2) (p < 0.05). PRF scaffold and ADSCs have the potential to serve as an effective biological wound dressing for burn wounds, accelerating the healing process and offering an alternative to traditional skin grafting.

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来源期刊
Cell and Tissue Banking
Cell and Tissue Banking CELL BIOLOGY-ENGINEERING, BIOMEDICAL
CiteScore
3.10
自引率
13.30%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Cell and Tissue Banking provides a forum for disseminating information to scientists and clinicians involved in the banking and transplantation of cells and tissues. Cell and Tissue Banking is an international, peer-reviewed journal that publishes original papers in the following areas: basic research concerning general aspects of tissue banking such as quality assurance and control of banked cells/tissues, effects of preservation and sterilisation methods on cells/tissues, biotechnology, etc.; clinical applications of banked cells/tissues; standards of practice in procurement, processing, storage and distribution of cells/tissues; ethical issues; medico-legal issues.
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