Aifang Zhou, Juan Chen, Xintong Dong, Pin Bai, Qianqian Zhu, Hong-Min Meng, Zhaohui Li
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Bimetallic CuRu nanozymes for colorimetric and smartphone-assisted rapid visual hypoxanthine biosensing in serum samples
Bimetallic Cu0.5Ru0.5 nanozymes were synthesized by alloying Ru with inexpensive Cu through a polyol method for hypoxanthine sensing. The prepared Cu0.5Ru0.5 nanozymes exhibit a peroxidase-like activity. Under the specific enzyme of xanthine oxidase (XOD), hypoxanthine was first converted to xanthine, and then to the final product uric acid, accompanied by the production of H2O2. Cu0.5Ru0.5 nanozymes could catalyze H2O2 to generate •OH and then oxidize colorless TMB to blue oxTMB, resulting in a visible color change. Therefore, the determination of hypoxanthine was achieved by monitoring the absorption value change of oxTMB at 652 nm. The proposed hypoxanthine sensing strategy displayed a high sensitivity and selectivity, with the limit of detection of 0.5 μM. Furthermore, we designed a rapid and convenient platform for hypoxanthine detection using a smartphone with image recognition and data processing function.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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