从北奥塞梯温泉中分离到的一种新的热敏水解细菌——骨芽孢杆菌科的骨芽孢菌,以及对骨芽孢菌属描述的修正

IF 4.2 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Olga A. Podosokorskaya , Maria I. Prokofeva , Alexandra A. Kuchierskaya , Alexandra A. Klyukina , Alexander G. Elcheninov
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引用次数: 0

摘要

从北奥塞梯(俄罗斯联邦)温泉中分离到一株新型厌氧适度嗜热细菌4138-strT。革兰氏阴性细胞不形成孢子,呈直或弯的丝状杆,偶有形成莲座。菌株在30 ~ 55℃、pH值6.1 ~ 8.7、NaCl值0 ~ 4%的条件下生长,在50℃、pH值7.1 ~ 7.5、NaCl值0.2 ~ 0.4%的条件下生长最佳。它是一种化学有机异养菌,生长在单糖(葡萄糖、麦芽糖、纤维素二糖等)和碳水化合物(淀粉、普鲁兰、层粘连蛋白、木聚糖、地衣聚糖、凝乳蛋白、球髓蛋白)或蛋白质底物(蛋白胨、色氨酸、明胶、酪蛋白)上。硫作为电子受体。葡萄糖发酵的主要产物是乙酸、氢和二氧化碳。主要细胞脂肪酸为iso-C15:0和前iso-C15:0。醌是MK-7。菌株4138-strT全基因组大小为3.275 Mbp;DNA G + C含量为42.1%。基因组分析允许鉴定编码碳水化合物活性酶和细胞外蛋白酶的基因。重建了菌株4138-strT的中枢代谢和发酵途径。基于16S rRNA基因序列和保守蛋白序列的系统发育分析,以及基于基因组的比较,菌株4138-strT形成了Tenuifilum属Tenuifilaceae (Bacteroidota门)的种水平谱系。本文提出了一新种骨骨Tenuifilum sp. nov.,类型菌株4138-strT(=KCTC 25386T = VKM B-3628T = UQM 41477T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tenuifilum osseticum sp. nov., a novel thermophilic hydrolytic bacterium within the Tenuifilaceae isolated from a North Ossetian thermal spring, and emended description of the genus Tenuifilum
A novel anaerobic moderately thermophilic bacterium, strain 4138-strT, was isolated from a thermal spring of North Ossetia (Russian Federation). Gram-negative cells were non-sporeforming, straight or curved filamentous rods, occasionally forming rosettes. The strain grew at 30–55 °C, pH range of 6.1–8.7, NaCl range of 0–4 %, with an optimum at 50 °C, pH 7.1–7.5 and 0.2–0.4 % NaCl. It was a chemoorganoheterotroph, growing on simple sugars (glucose, maltose, cellobiose, etc.) and carbohydrates (starch, pullulan, laminarin, xylan, lichenan, curdlan, pachyman) or proteinaceous substrates (peptone, tryptone, gelatin, casein). Sulfur was used as electron acceptor. Major products of glucose fermentation were acetate, hydrogen, and carbon dioxide. Major cellular fatty acids were iso-C15:0 and anteiso-C15:0. The quinone was MK-7. The size of the whole genome of strain 4138-strT was 3.275 Mbp; DNA G + C content was 42.1 %. Genome analysis allowed to identify genes encoding carbohydrate-active enzymes and extracellular proteases. In addition, central metabolism and fermentation pathways of strain 4138-strT were reconstructed. According to both phylogenetic analyses, based on 16S rRNA gene sequences and conserved protein sequences, as well as genome-based comparisons, strain 4138-strT formed a species-level lineage within Tenuifilum genus of Tenuifilaceae family (phylum Bacteroidota). Here we propose a novel species Tenuifilum osseticum sp. nov. with type strain 4138-strT(=KCTC 25386T = VKM B-3628T = UQM 41477T).
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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