组织蛋白酶F影响烟草粉虱对TYLCV和ToCV的病毒获取、保留和传播。

Xiaobin Shi, Pei Wang, Caihua Shi, Ruixue Luo, Deyong Zhang, Zhuo Zhang, Yang Gao, Jing Peng, Evan L Preisser, Yong Liu
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引用次数: 0

摘要

番茄黄卷叶病毒(TYLCV)和番茄褪绿病毒(ToCV)是主要由烟粉虱传播的侵染植物的病毒。除了对农业生产产生重大的个体影响外,TYLCV和ToCV共同感染也越来越普遍,并可能对番茄和其他作物造成毁灭性损失。组织蛋白酶是溶酶体中的主要蛋白酶,它既影响免疫反应,也影响植物蛋白的消化,可能有助于介导Bemisia-virus-plant相互作用。我们研究了组织蛋白酶在烟粉虱与植物病毒TYLCV和ToCV单独和联合相互作用中的作用。摄食tylcv感染、tocv感染和共感染植物后,烟粉虱体内组织蛋白酶F水平急剧升高,并在摄食停止后数天内保持升高。在所有病例中,合并感染的烟粉虱MED的水平高于单独感染的个体。联合感染的两种病毒的病毒载量也比单独感染的高,这表明TYLCV和ToCV之间存在协同关系。接下来,我们研究了使用组织蛋白酶抑制剂、诱导剂或对照给药如何影响病毒的获取、保留和传播。与对照组相比,组织蛋白酶诱导剂处理的烟粉虱感染病毒的获取和滞留量较低;组织蛋白酶抑制剂处理的烟粉虱具有较高的病毒获得率和病毒滞留率。病毒传播在抑制剂处理中最高,在诱导剂处理中最低。我们的结果为设计新的控制策略来管理昆虫媒介及其传播的病毒提供了更多的机会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cathepsin F alters viral acquisition, retention, and transmission of TYLCV and ToCV by Bemisia tabaci MED.

Tomato yellow leaf curl virus (TYLCV) and Tomato chlorosis virus (ToCV) are plant-infecting viruses that are mainly transmitted by Bemisia tabaci Gennadius. In addition to their significant individual impacts on agricultural production, TYLCV and ToCV co-infections are increasingly common and can cause devastating losses in tomato and other crops. Cathepsins, the main proteases in lysosomes, affect both immune responses and the digestion of plant proteins and may help mediate Bemisia-virus-plant interactions. We conducted research exploring the role of cathepsin in the interaction between B. tabaci MED and the plant viruses TYLCV and ToCV, both singly and in combination, on tomato. Levels of cathepsin F increased sharply in B. tabaci MED after feeding on TYLCV-infected, ToCV-infected, and co-infected plants and remained elevated for several days after feeding cessation. In all cases, levels were higher in co-infected B. tabaci MED than in singly infected individuals. Viral loads of each virus were also higher in co- versus singly infected B. tabaci MED, suggesting a synergistic relationship between TYLCV and ToCV. We next studied how dosing B. tabaci MED with a cathepsin inhibitor, inducer, or control affected viral acquisition, retention, and transmission. Viral acquisition and retention were lower in B. tabaci MED treated with cathepsin inducer than in controls; B. tabaci MED treated with cathepsin inhibitor had higher rates of viral acquisition and retention. Viral transmission was highest in the inhibitor treatment and lowest in the inducer treatment. Our results provide more opportunities for the design of novel control strategies to manage insect vectors and their transmitted viruses.

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