Subcellular dynamics in unicellular parasites.

Bioimaging has made tremendous advances in this century. Innovations in high- and super-resolution microscopy are well established, and live-cell imaging is extensively used to gain an overview of dynamic processes. But the combination of high spatial and temporal resolution necessary to capture intracellular dynamics is rarely achieved. Further, efficient software pipelines - that can handle the recorded data and allow comprehensive analyses - are being developed but lag behind other technical innovations in applicability for broad groups of researchers. Especially in parasites, which offer great potential for studying subcellular dynamics, the possibilities have only begun to be probed. In all cases, the complete description of dynamic molecular movement in 3D space remains a challenging necessity.