An octahedral metal oxide nanoparticle-based dual-signal sensing platform for simultaneous detection of histidine and lysine in human blood plasma and urine

Histidine and lysine serve as essential amino acids in physiological processes and biomarkers for specific diseases, requiring precise detection methods in a variety of samples. This study presents an affordable single colorimetric probe that employs nickel oxide nanoparticles (NiONPs) as an artificial enzyme to detect histidine and lysine, improving conventional analytical limitations. The characterization of NiONPs was executed using SEM-EDX, FE-SEM, FTIR and XRD. The NiONPs demonstrated peroxidase-like catalytic activity on the conversion of TMB to oxidized TMB (oxTMB) in the presence of H₂O₂, utilizing optimization parameters like pH value (3), TMB concentration (10 mM), H₂O₂ concentration (60 mM), and incubation time (18 min). The study revealed that Ni and O atoms are present on the surface of NiONPs, allowing for specific interactions with essential amino acids and temporarily hindering the catalytic activity of oxidized TMB. The method exhibited a low limit of detection (LOD) of 0.07 μM (10–100 μM) for histidine and 1.1 μM (15–150 μM) for lysine with good stability. The proposed strategy was validated with urine and plasma samples, yielding favorable recoveries of 93.6–98.2% in urine and 90.5–96.0% in plasma for histidine and 91.2–94.8% in urine and 88.4–93.3% in plasma for lysine, supporting its selectivity, feasibility, and reliability for practical applications. In the future, this methodology will facilitate the integration of histidine and lysine detection into microfluidic systems using NiONPs as a colorimetric probe.