miR-27a-3p多态性与种植体周围炎易感性的相关性：一项病例对照研究

IF 3.2 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International dental journal Pub Date : 2025-02-10 DOI:10.1016/j.identj.2025.01.013

Xiaojie Gao , Yanan Ha

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引用次数: 0

摘要

背景：种植体周围炎（PI）是一种慢性炎症反应，影响种植体周围的软组织和骨组织。炎症状况，加上骨代谢的改变，可能是由miR-27a-3p调节的。目的：本研究旨在探讨miR-27a-3p rs895819多态性与PI易感性的关系及其对炎症因子的影响和机制。方法：采用逆转录聚合酶链反应（RT-PCR）检测miR-27a-3p的相对水平。采用限制性内切片段长度多态性对Rs895819进行基因分型。酶联免疫吸附试验（ELISA）试剂盒检测RUNX1、c反应蛋白（CRP）和白细胞介素-6 （IL-6）的浓度。从ENCORI、miRDB、TargetScan等数据库中鉴定出miR-27a-3p重叠的靶基因，便于miR-27a-3p靶基因的选择。重叠基因通过维恩图进行编译。目的基因的功能富集分析通过基因本体（GO）、京都基因与基因组百科全书（KEGG）和蛋白质-蛋白质相互作用（PPI）网络进行。结果：在PI患者中发现miR-27a-3p低表达。GG基因型(粗比值比(OR) = 2.898, 95%可信区间(CI) = 1.419-5.917；校正后：OR = 2.157, 95% CI = 1.045-4.455)和G等位基因（P = 0.003, OR = 1.501, 95% CI = 1.144-1.968）与PI易感性增强有显著相关性。从这些数据库中确定了直接靶基因RUNX1。具有rs895819 GG基因型的PI患者miR-27a-3p水平降低，RUNX1 （P < 0.05）、CRP （P < 0.001）、IL-6 （P < 0.001）和白细胞（WBC）计数浓度升高（P < 0.05）。结论：这些结果提示miR-27a-3p的rs895819是PI患者的重要风险预测因子。该变异与PI患者miR-27a-3p水平降低、RUNX1水平升高和炎症因子水平升高相关。

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Correlation Between miR-27a-3p Polymorphisms and Peri-Implantitis Susceptibility: A Case-Control Study

Background

Peri-implantitis (PI) is characterised by a chronic inflammatory response that affects both the soft and bone tissues around dental implants. Inflammatory condition, coupled with alterations in bone metabolism, may be modulated by miR-27a-3p.

Aim

This research aims to investigate the relationship between miR-27a-3p rs895819 polymorphism with PI susceptibility, along with its effects and mechanism on inflammatory factors.

Methods

Relative miR-27a-3p level was assessed using reverse transcription-polymerase chain reaction (RT-PCR). rs895819 was genotyped by restriction fragment length polymorphism. Enzyme-linked immunosorbent assay (ELISA) kits detected the concentrations of RUNX1, C-reactive protein (CRP), and interleukin-6 (IL-6). Overlapping target genes of miR-27a-3p were identified from databases, including ENCORI, miRDB, and TargetScan databases, which facilitated the selection of target genes of miR-27a-3p. Overlap genes were compiled by the Venn diagram. Functional enrichment analysis for target genes was performed via Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein-Protein Interaction (PPI) networks.

Results

Underexpressed miR-27a-3p was discovered in PI patients. GG genotype (crude: odds ratio (OR) = 2.898, 95% confidence interval (CI) = 1.419-5.917; adjusted: OR = 2.157, 95% CI = 1.045-4.455) and G allele (P = .003, OR = 1.501, 95% CI = 1.144-1.968) of rs895819 demonstrates a significant association with an enhanced PI susceptibility. The direct target gene RUNX1 has been identified from these databases. PI patients possessing rs895819 GG genotype exhibit diminished level of miR-27a-3p and elevated concentrations of RUNX1 (P < .05), CRP (P < .001), IL-6 (P < .001), and white blood cell (WBC) count (P < .05).

Conclusion

These results suggest that rs895819 of miR-27a-3p serves as a significant risk predictor for PI patients. This variant is associated with decreased miR-27a-3p levels, alongside elevated RUNX1 levels and inflammatory factor levels in PI patients.

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来源期刊

International dental journal 医学-牙科与口腔外科

CiteScore

4.80

自引率

6.10%

发文量

159

审稿时长

63 days

期刊介绍： The International Dental Journal features peer-reviewed, scientific articles relevant to international oral health issues, as well as practical, informative articles aimed at clinicians.